Page 15 - LC-SFC_Pharma_Brochure

C190-E211

Technical Ultra Fast Method Scouting (2)

Report —Maximizing the Efficiency of Method Development

Akihiro Kunisawa , Yusuke Osaka , Daiki Fujimura , Satoru Watanabe , Shinichi Kawano 1
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Abstract:

Due to the large amounts of time required for determining analytical conditions (method scouting), users are looking for ways to minimize
the time required for method scouting.
This report describes an example of using a Nexera Method Scouting system in combination with dedicated method scouting software to
determine analytical conditions for simultaneous analysis of nine types of sulfa drugs. The dedicated method scouting software, Method
Scouting Solution, is able to use the multi-data report functionality in LabSolutions˜ DB/CS software to rapidly ÿnd optimal analytical
conditions from huge amounts of analytical results. Therefore, this report also describes the multi-data report function.
Keywords: Nexera Method Scouting, Method Scouting Solution, LabSolutions DB/CS, Multi-Data Report

1. Introduction
1. Intr oduction
Developing analytical methods for new compounds mainly involves The Nexera Method Scouting system is based on the Nexera˜ X2
four steps. ultra high performance liquid chromatograph, which features a
(1) Simulation 130˜ MPa pressure tolerance, and is used for applications corre-
Retention behavior is predicted of˛ine ba sed on information about the sponding to Step˜2 on the left. The system is able to comprehen-
structural and other properties of target compounds being analyzed. sively search for analytical conditions by automatically trying up
to 192 combinations of eight types of mobile phases and twelve
(2) Method Scouting types of columns.
Column and mobile phase candidates are comprehensively scouted
by performing trial analyses using different combinations of various This report describes an example of using the Nexera Method
columns and mobile phases. Scouting system, Method Scouting Solution software, and
multi-data report functionality included in LabSolutions˜ DB/CS
(3) Method Optimization software to determine optimal analytical conditions by compar-
The columns and mobile phases selected during method scouting ing results measured using a variety of analytical conditions.
are used to optimize various parameters.
(4) Method Validation
This step evaluates the robustness of analytical methods.
Reservoir switching Solvent delivery pump
valve
Flow line switching value
(FCV-34AH)
Photodiode
Mixer array detector

Auto
sampler
Solvent delivery Columns Manifold
pump
Column oven

Fig. 2 Flow Line Diagram of Nexera Method Scouting System
Fig. 1 Nexera Method Scouting System (Shown with Six Types of Columns)

1 Analytical & Measuring Instruments Division 1