Page 27 - Pharmaceutical Solution for Pharma Analysis

LC-15-ADI-036

To ensure reliable fractionation and purification of precious samples, the Purification Solution software
offers three fractionation modes

Automatic Fractionation Mode
In this mode, the software automatically identifies peaks and collects fractions
based on parameter settings.

Manual Fractionation Mode
In this mode, the mouse pointer is used to fractionate peaks while viewing the
window. When the same sample is concentrated by repeated injections, the first
fractionation range is saved then the second and subsequent samples are
automatically fractionated using the same fractionation range.

Time-Specified Fractionation Mode
This mode collects fractions based on the retention times in previously acquired
data. It is ideal for routinely performed preparative purification processes.

Conclusion

The Prominence UFPLC seamlessly integrates traditional Prep LC with novel fraction trapping for upto
five compounds of interest. The instrument is controlled by a dedicated walk-up software designed to
empower non-expert users to easily set conditions for chromatographic separation and isolation of
target compounds, trapping, purifying, eluting and collecting highly purified compounds in as little as
90mins. For applications involving the isolation of low concentration targets, repeated injection and
collection to the same trapping column to increase the amount of compound trapped on column prior
to elution is easily accomplished.

The Prominence UFPLC eliminates some of the problems associated with conventional Prep LC, especially
poor purity of collected compounds due to mobile phase additives, which become contaminants in the
final collected fraction and inhibit powderization. Shimadzu’s “Shim-pack C2P-H” trap column strongly
retains target compounds allowing unwanted organic solvents, water and additives to be flushed away in
very quick time.

First Edition: March. 2018

Shimadzu Analytical (India) Pvt.Ltd.
1 A/B, Rushabh Chambers, Makwana Road,
Marol, Andheri (East), Mumbai- 400059, India.
Tel: +91 22 29204741 Fax: +91 22 29205679
www.shimadzu.in