Page 49 - Clinical-Medical Solution for Clinical Chemistry

Integration of amino acid, acylcarnitine and steroids
analysis in single FIA/LC-MS/MS platform

Introduction

Analysis of amino acids (AA) and acylcarnitines (AC) in The use of LC/MS/MS results in a reduction of false
dried blood spot (DBS) sample collection method by positives and a more accurate quantitative
ow injection analysis (FIA) is now widely used. On the performance. The requirements against steroid analysis
other hand, traditionally, analysis of steroid such as by LC/MS/MS are getting more stringent issues. In this
17-hydroxyprogesterone is done by immunoassays but study, we present a strategy for performing both
LC/MS/MS will be an attractive analytical alternative AA/AC and steroids analysis within a single LC/MS/MS
because it can also screen for other related steroids. platform.

Methods and Materials

The experimental setup designed to combine a FIA measurement covers 8 AAs and 17 ACs and a LCMS
measurement for 5 steroids includes cortisol, 21-deoxycortisol (21-DOF), 11-deoxycortisol (11-DOF),
androstenedione (4-AD) and 17-hydroxyprogesterone (17-OHP) with 2 position 6 port high pressure valve.

AAs, ACs Guard Column

SIL-30AC
LC-30AD 2 1
LCMS (LCMS)
3 6
FCV-12AH
4 5

Column
FCV-12AH

Guard
Steroids Guard Column
Column Column
(SIL-30AC)
SIL-30AC
LC-30AD 2 1
LCMS
3 6
FCV-12AH
4 5

Column

Figure 1 Flow Diagram of FIA/LC-MS/MS system

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