Page 54 - Clinical-Medical Solution for Clinical Chemistry
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Integration of amino acid, acylcarnitine and steroids
analysis in single FIA/LC-MS/MS platform
Conc. LOD LOQ Conc. LOD LOQ
(ng/mL) Area %RSD S/N (ng/mL) (ng/mL) (ng/mL) Area %RSD S/N (ng/mL) (ng/mL)
5 15,919 6.05 66.1 0.23 0.76 5 97,910 0.62 184.1 0.08 0.27
25 54,064 2.11 246.3 25 387,155 0.92 946.5
75 186,970 0.85 681.0 75 1,247,236 0.08 2338.6
Cortisol 4-AD
125 322,782 1.68 971.9 125 2,187,611 0.26 5318.0
150 345,172 0.26 994.9 150 2,406,566 0.36 4459.4
500 1,307,317 0.16 1606.4 500 9,143,414 0.69 36564.1
5 23,687 1.87 27.8 0.54 1.80 5 40,107 6.12 79.3 0.19 0.63
25 98,627 3.64 103.0 25 157,773 2.85 366.5
75 388,221 2.04 473.1 75 504,027 1.03 830.9
21-DOF 17-OHP
125 701,690 1.16 181.9 125 874,209 1.56 3824.8
150 758,860 0.43 537.1 150 951,763 0.62 1635.5
500 3,000,289 0.70 214.5 500 3,694,638 0.20 1932.9
5 39,161 1.53 60.5 0.25 0.83
25 167,525 2.43 218.1
75 534,404 0.39 436.3
11-DOF
125 964,525 0.26 94.2
150 1,029,424 0.53 695.0
500 3,872,397 0.60 97.9
Conclusions
This platform is an effective tool for an initial screening and able to minimize sample consumption down to 1 uL.
However, the use of a high performance LC/MS/MS system is highly recommended in order to achieve the appropriate
level of sensitivity for the steroids especially. Using high performance LC/MS/MS (LCMS-8050, Shimadzu Corporation,
Japan), the observed limit of detection (LOD) for the analysis of 17-hydroxyprogesterone was 0.19 ng/mL.
First Edition: May, 2015
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