Page 51 - Clinical-Medical Solution for Clinical Chemistry

Integration of amino acid, acylcarnitine and steroids
analysis in single FIA/LC-MS/MS platform

Mass (LCMS-8050 triple quadrupole mass spectrometry)
Ionization : heated ESI
Nebulizing Gas Flow : 3 L / min Drying Gas Pressure : 10 L / min
Heating gas ow : 10 L/min DL Temperature : 250 ºC
BH Temperature : 500 ºC Interface Temperature : 400 ºC
MRM parameter :
Target Q1>Q3 IS Q1>Q3 Target Q1>Q3 IS Q1>Q3
Phe 166.10>120.10 - Phe IS 172.10>126.10 C5 246.20>85.00 - C5 IS 255.20>85.00
Leu 132.10>86.10 - Leu IS 135.10>89.10 C5DC 276.10>85.00 - C5DC IS 279.10>85.00
Met 150.10>104.10 - Met IS 153.10>107.10 C5OH 262.20>85.00 - C5OH IS 265.20>85.00
Tyr 182.10>136.10 - Tyr IS 188.10>142.10 C6 260.20>85.00
- C8 IS 291.20>85.00
Val 118.10>72.10 - Val IS 126.10>80.10 C8 288.20>85.00
Cit 176.10>113.10 - Cit IS 178.10>115.10 C10 316.20>85.00
- C12 IS 347.30>85.00
Arg 175.10>70.10 - Arg IS 180.10>75.10 C12 344.30>85.00
Ala 90.00>44.00 - Ala IS 94.00>48.00 C14 372.30>85.00 - C14 IS 381.30>85.00
C0 162.10>103.00 - C0 IS 171.10>103.00 C16 400.30>85.00
- C16 IS 403.30>85.00
C2 204.10>85.00 - C2 IS 207.10>85.00 C16OH 416.30>85.00
C3 218.10>85.00 - C3 IS 221.10>85.00 C18 428.40>85.00
- C18 IS 431.40>85.00
C4 232.20>85.00 C18OH 444.40>85.00
- C4 IS 235.20>85.00
C4OH 248.20>85.00
R.T(min) Q1>Q3 CE (V) R.T(min) Q1>Q3 CE (V)
363.15>121.10 -31 365.15>122.10 -27
Cortisol 1.50 Cortisol-D2 1.50
363.15>327.10 -17 365.15>329.10 -18
347.15>311.15 -15 355.20>319.25 -18
21-DOF 1.85 21-DOF-D8 1.82
347.15>121.10 -28 355.20>46.10 -45
347.15>109.10 -31 349.20>109.05 -33
11-DOF 2.20 11-DOF-D2 2.23
347.15>97.05 -27 349.20>97.10 -29
287.15>97.05 -24 290.15>100.00 -22
4-AD 2.65 4-AD-13C3 2.65
287.15>109.10 -24 290.15>112.00 -28
331.15>109.05 -29 339.20>100.10 -27
17-OHP 3.02 17-OHP-D8 3.00
331.15>97.10 -30 339.20>113.10 -31

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