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Application No.C139
News

Q Measurement Results Table 3 Example of Enzymatic Activity Measurement Results
The enzymatic activities of IDUA, GLA, and GAA were IDUA GLA GAA
measured. Examples of enzymatic activity measurement (μmol/h/L) (μmol/h/L) (μmol/h/L)
results are shown in Table 3 and MRM chromatograms Blank 0.3 0.1 0.1
of each target compound in Fig. 4. Filter paper without Control 15.2 10.1 6.0
any blood was used as blanks. Sample A is a sample Sample A 13.2 6.6 7.3
that contains enzymes with each activity, Sample B is Sample B 1.8 6.9 5.8
missing IDUA, Sample C is missing GLA, and Sample D Sample C 17.6 0.5 9.8
is missing GAA. Whereas peaks were clearly detected Sample D 8.4 2.0 0.8
for enzyme decomposition products in Sample A, there
was a decrease in target peaks in Samples B to D. Activity (μmol/h/L) = [(P/IS) × [IS] × 30/3.4] / 22
· (P/IS): Area ratio between product (P) and internal standard (IS)
· [IS]: Concentration (μmol/L) of internal standard (IS)
· 30: Volume of solution added (μL)
· 3.4: Volume of blood in DBS (μL)
· 22: Reaction time (hr)

IDUA GLA GAA
(×100,000) (×1,000,000) (×1,000,000)
2.0 Blank Blank 4.0 Blank
3.0
1.5 3.0
2.0
1.0 2.0
IDUA-IS IDUA-P
0.5 1.0 GLA-P 1.0 GAA-IS GAA-P
GLA-IS
0.0 0.0 0.0
0.0 1.0 2.0 3.0 4.0 min 0.0 1.0 2.0 3.0 4.0 min 0.0 1.0 2.0 3.0 4.0 min
(×100,000) (×1,000,000) (×1,000,000)
Positive Positive Positive
2.0
IDUA-P 4.0 GAA-P
Control 3.0 GLA-P Control Control
1.5 3.0
2.0
1.0 2.0
1.0
0.5 1.0
IDUA-IS GLA-IS GAA-IS
0.0 0.0 0.0
0.0 1.0 2.0 3.0 4.0 min 0.0 1.0 2.0 3.0 4.0 min 0.0 1.0 2.0 3.0 4.0 min
(×100,000) (×1,000,000) (×1,000,000)
2.0 Sample A GLA-P Sample A 4.0 GAA-P Sample A
IDUA-P
3.0
1.5 3.0
2.0
1.0 2.0
1.0
0.5 1.0
IDUA-IS GLA-IS GAA-IS
0.0 0.0 0.0
0.0 1.0 2.0 3.0 4.0 min 0.0 1.0 2.0 3.0 4.0 min 0.0 1.0 2.0 3.0 4.0 min
(×100,000) (×1,000,000) (×1,000,000)
2.0 Sample B Sample C 4.0 Sample D
3.0
1.5 3.0
2.0
1.0 2.0
IDUA-P
0.5 1.0 GLA-P 1.0 GAA-P
IDUA-IS GLA-IS GAA-IS
0.0 0.0 0.0
0.0 1.0 2.0 3.0 4.0 min 0.0 1.0 2.0 3.0 4.0 min 0.0 1.0 2.0 3.0 4.0 min
Fig. 4 MRM Chromatograms of Each Target Compound
Blank: Sample filter paper containing no blood; Sample A: Sample containing enzymes with all activities;
Sample B: Sample missing IDUA; Sample C: Sample missing GLA; and Sample D: Sample missing GAA
[References]
la Marca G et al., Anal. Chem. 81 (2009) 6113-6121
Ombrone D. et al., Eur. J. Mass Spectrom. 19, 497-503 (2013)
[Acknowledgments]
This Application News bulletin was prepared based on guidance and samples provided by Dr. G. la Marca (Meyer Children's Hospital, Mass
Spectrometry, Clinical Chemistry and Pharmacology Laboratory, Florence, Italy). We are sincerely grateful for his help.
Notes: The equipment mentioned in this article has not been approved/certified as medical equipment under Japan‘s Pharmaceutical and Medical
Device Act.
The analytical methods described in this article cannot be used for diagnostic purposes.
First Edition: Dec. 2016
For Research Use Only. Not for use in diagnostic procedure.
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