Page 45 - Clinical-Medical Solution for Clinical Chemistry

Determination of Unbound Urinary Amino Acids Incorporated
with Creatinine Normalization by LC-MS/MS Method with
CLAM-2000 Online Sample Pre-treatment

Conclusions

A fully-automated method for quantitative analysis of 22 amino acids (AA) and creatinine (CRE) in urines was
established on a novel platform of CLAM-LC-MS/MS. An Intrada Amino Acid column adopted allows the analysis of
AA without derivatization by LC/MS/MS. Twenty-eight urine specimens from health volunteers were analysed and the
results were used to calculate total creatinine-normalized AA (TCNA), from which two distinct groups of urines are
identi ed. Multivariate analysis of the AA pro les of the 28 specimens con rms the signi cant importance of the
classi cation based on TCNA, which may provide a new approach in characterization of AA pro les linking with
diagnosis of disease and health conditions.

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Disclaimer: The LCMS-8040, CLAM-2000 and the contents are intended for Research Use Only (RUO). Not for use
in diagnostic procedures.

First Edition: June, 2017

© Shimadzu Corporation, 2017