Page 41 - Clinical-Medical Solution for Clinical Chemistry

Determination of Unbound Urinary Amino Acids Incorporated
with Creatinine Normalization by LC-MS/MS Method with
CLAM-2000 Online Sample Pre-treatment

Table 2: Summary of MRM quanti cation method and performance for analysis of 22 AA and CRE on CLAM-2000
coupled with LC-MS/MS: calibration range, linearity, LOQ, accuracy and repeatability (%RSD)

RT MRM transition IS Calibration Curves and Quantitation Performance
No. Name
(min) (m/z) Range (µM) R 2 LOQ (µM) Accuracy (%) %RSD (n=6)
1 Trp 3.39 205.10>188.20 0.1-100 0.998 0.14 93.5 2.1
2 Phe 3.67 166.10>120.10 0.1-100 0.999 0.12 99.4 1.6
3 Tyr 3.99 182.10>136.20 0.5-100 0.996 0.78 89.3 0.7
4 Leu 4.62 132.10>86.30 0.1-100 0.998 0.14 98.2 1.5
5 Met 4.81 150.10>56.10 0.1-100 0.999 0.11 104.5 1.6
6 Ile 5.11 132.10>86.30 0.1-100 0.998 0.10 97.7 1.0
7 Val 6.06 118.20>72.05 0.5-100 0.997 0.26 98.8 2.8
8 Glu 7.01 148.10>84.10 0.1-100 0.996 0.12 101.2 2.3
9 Pro 7.26 116.10>70.10 0.1-100 0.999 0.07 104.6 2.2
10 Thr 7.62 120.10>74.00 1-100 0.997 0.9 103.0 4.3
11 Asp 7.81 134.10>73.90 5-100 0.994 5.0 102.5 4.6
12 Ala 8.16 90.10>44.10 1-100 0.995 1.5 103.4 3.3
13 Ser 8.91 106.10>60.20 1-100 0.999 1.6 97.2 3.3
14 Gln 9.10 147.10>84.10 0.1-100 0.999 0.1 106.6 11.8
15 Gly 9.30 76.1 (SIM) 2-100 0.998 4.6 100.3 6.7
16 Asn 9.38 133.10>74.10 0.5-100 0.999 0.66 99.3 3.4
17 Cit 9.98 176.00>70.10 0.1-100 0.997 0.10 101.2 4.6
18 CRE 10.08 114.00>44.00 0.1-100 0.996 0.12 99.3 2.6
19 Cys 12.13 241.00>151.95 0.1-100 0.999 0.12 99.1 8.4
20 His 16.52 156.10>110.10 2-50 0.991 2.2 103.2 7.4
21 Lys 16.95 147.00>84.10 0.5-50 0.996 0.5 109.0 12.9
22 Orn 17.21 133.10>70.05 0.5-50 0.995 0.7 113.3 16.2
23 Arg 17.97 175.10>70.10 0.5-50 0.993 0.3 105.5 8.6
Notes: (1) LOQ values refer to the AA concentrations in neat solution; (2) Accuracy and repeatability values refer to 10 µM level

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