Page 57 - Clinical-Medical Solution for Clinical Chemistry

A Novel Platform of On-line Sample Pre-treatment and LC/MS/MS
Analysis for Screening and Quantitation of Illicit Drugs in Urine

Table 1: Analytical conditions on LCMS-8040
Column : Biphenyl 2.6µ, 100A (100 mmL x 2.10mm I.D.)
Mobile Phase : A: Water with 0.1% FA
B: Methanol with 0.1% FA
Elution Program : Gradient elution (11.0 minutes)
B: 3% (0 to 0.5 min) → 90% (5.5 to 7.0 min) → 3% (7.5 to 11.0 min)
Flow Rate : 0.4 mL/min
Oven Temp. : 40ºC
Injection : 5 µL
Interface : ESI
MS Mode : MRM, Positive
Block Temp. : 400ºC
DL Temp. : 250ºC
CID Gas : Ar, 270 kPa
Nebulizing Gas : N 2, 2.0 L/min
Drying Gas : N 2, 15.0 L/min

MeOH wetting (conditioning)

Add 20uL of Sample (urine)

Add 20uL of I.S.

Add 40uL of ACN/MeOH

Vortex for 60sec

Vacuum Filter for 90sec

Transferring to LC/MS/MS

Figure 2: Typical auto-work ow of urine sample via protein-crash
and adding IS for LC/MS/MS by CLAM-2000

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