Page 61 - Clinical-Medical Solution for Clinical Chemistry

A Novel Platform of On-line Sample Pre-treatment and LC/MS/MS
Analysis for Screening and Quantitation of Illicit Drugs in Urine

Table 3: MRM quantitation method of eighteen illicit drugs
Accuracy (%) *Cut Off Avg. P.E
Compd. R2
20 ng/mL 50 ng/mL 200 ng/mL ng/mL (%)
NIME 93.7 103.0 99.9 0.9997 5 99.1
KET 117.5 91.6 100.3 0.9983 100 107.9
NORKET 91.6 104.0 99.8 0.9996 100 106.5
BU 94.5 102.7 99.9 0.9998 2 118.1
NORBU 112.8 93.9 100.3 0.9991 2 183.0
MORP 108.7 95.8 100.2 0.9995 300 62.2
COD 96.0 101.9 99.9 0.9999 300 88.8
MAM 89.6 105.0 99..8 0.9994 10 139.5
BE 102.5 98.8 100.1 0.9999 150 111.3
PCP 92.0 103.8 99.8 0.9996 25 88.3
METQ 80.8 109.2 99.6 0.9980 250 101.9
METD 130.2 85.5 100.6 0.9951 250 161.6
AMPH 91.7 104.0 99.8 0.9996 200 92.3
MDEA 84.0 107.7 99.7 0.9986 200 84.5
MDA 92.1 103.8 99.8 0.9996 200 80.8
MDMA 110.4 95.0 100.2 0.9994 200 98.5
PROP 102.2 98.9 100.0 0.9999 300 122.1
METH 83.7 107.8 99.7 0.9985 200 84.0
*The Cut Off is based on European Guidelines for Workplace Drug Testing in Urine

1000000 1000000
900000 (A) 900000 (B) Methadone
800000 800000 Phencyclidine (PCP)
700000 700000 Propoxyphene
600000 600000
500000 500000 Methaqualone
400000 400000 Methamphetamine MDMA Benzoylecgonine (BE)
300000 300000 Amphetamine MDEA Norketamine Ketamine Nimetazepam
200000 200000 Norbuprenorphine Buprenorphine
Morphine Codeine
100000 100000 MDA MAM
0 0
3.0 4.0 5.0 6.0 min 3.0 4.0 5.0 6.0 min

Figure 5: Total MRM chromatograms of (A) blank urine and (B) spiked urine with eighteen illicit drugs (200 ng/mL).

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