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Application No.C142$
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Q Distinguishing Norfenefrine and Octopamine in Urine
Norfenefrine is a positional isomer of octopamine resulting in the same retention time and MRM transitions for the
unchanged parent drug molecule. However, by detecting the corresponding sulphate metabolite using rapid polarity
switching enabled a positive identification.

Urine: norfenefrine-administered (×10 dilution)
Unchanged drug Sulphate (Positive ion) Sulphate (Negative ion)
(×100,000) (×100,000) (×1,000,000)
1.00 5.0 4.0
154.20 > 91.25 (+) 234.20 > 136.20 (+) 232.20 > 152.20 (-)
154.20 > 65.25 (+) 4.0 234.20 > 91.20 (+) 232.20 > 121.15 (-)
0.75 3.0
XICs of 0.50 3.0 2.0
Norfenefrine 2.0
0.25 1.0
1.0
0.00 0.0 0.0
5.5 6.0 min 5.5 6.0 min 5.5 6.0 min
(×100,000) (×100,000)
1.00 1.00
154.20 > 91.25 (+) 232.20 > 134.15 (-)
154.20 > 119.20 (+) 232.20 > 107.10 (-)
0.75 0.75
XICs of Norfenefrine
sulphate
0.50 0.50 Octopamine
Octopamine
sulphate
0.25 0.25
0.00 0.00
5.5 6.0 min 5.5 6.0 min
Same RT’s and MRM’s Norfenefrine sulphate was positively identified
for both compounds using negative ion detection
Urine: octopamine -administered (×10 dilution)
Unchanged drug Sulphate (Positive ion) Sulphate (Negative ion)
(×1,000) (×10,000) (×100,000)
7.5 5.0
154.20 > 91.25 (+) 234.20 > 136.20(+) 232.20 > 152.20 (-)
154.20 > 65.25 (+) 4.0 234.20 > 91.20(+) 7.5 232.20 > 121.15 (-)
5.0
3.0 5.0
XICs of 2.0
2.5
Norfenefrine 1.0 2.5
0.0 0.0 0.0
5.5 6.0 min 5.5 6.0 min 5.5 6.0 min
(×1,000) (×1,000,000)
154.20 > 91.25 (+) 1.5 232.20 > 134.15 (-)
154.20 > 119.20 (+) 232.20 > 107.10 (-) Octopamine
XICs of 5.0
1.0 sulphate
Octopamine Norfenefrine
2.5 0.5 sulphate
0.0 0.0
5.5 6.0 min 5.5 6.0 min
Same RT’s and MRM’s Octopamine sulphate was positively identified
for both compounds using negative ion detection

Fig. 6 Results of Urine: Norfenefrine and Octopamine were separately administered
The sample used for this analysis was provided by Anti-Doping Laboratory, LSI Medience Corporation, Tokyo, Japan
References: Anal Bioanal. Chem. (2015), 407, 5354-5379
Drug Test. Analysis (2015), 7, 973–979
Notes: ɾ The products mentioned in this article have not received approval for use as medical devices based on the Pharmaceutical and Medica Device
Act.
ɾ The analytical methods mentioned in this article cannot be used for diagnostic purposes, for Research Use Only (RUO).

First Edition: Dec. 2016
Second Edition: Jan. 2017
For Research Use Only. Not for use in diagnostic procedure.
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