Page 67 - Clinical-Medical Solution for Clinical Chemistry

LAAN-A-LM-E128

Application Direct Probe Ionization Mass Spectrometer

News
15 Second Screening Analysis of Cyanide in Blood
Serum Without Pretreatment
No. C158

Recent years have witnessed an increasing trend in  Analysis Sequence Without Pretreatment
incidents of crime and poisoning involving various legal Potassium cyanide was added to blood serum to obtain a
drugs and toxic substances. The diversity in the types of final concentration of 10 μg/mL and then taurine and
used substances has lead to such incidents becoming a naphthalene dialdehyde were added to perform
social problem. In the fields of forensic medicine, forensic derivatization. The obtained cyanide derivative (Fig. 2)
*1
toxicology, and critical care, finding and identifying was added to blood serum and used as the sample.
causative agents is a problem that requires establishment
of an analysis method that is both quick and highly While complex pretreatment, such as that shown in Fig. 3,
sensitive. There is also growing demand in these is required in conventional blood serum analysis, analysis
workplaces to further simplify the complex pretreatment that utilizes PESI can be performed using blood serum that
processes and instrument operations as well as to increase contains cyanide derivatives either as-is or diluted with
analysis speed. While various analysis instruments have water by injecting it onto a small (10 μL) sample plate and
been utilized until now for analyzing specific components setting the sample plate in the instrument.
in blood, most instruments require complex pretreatment,
such as extracting the target component from blood.
What is needed is a screening method that best reduces
the time and labor required to perform analysis.
Probe electrospray ionization (PESI) is a direct ionization
technique that ionizes sampled target components by
sampling samples using an ultrafine and minimally
invasive probe and applying high voltage to the probe tip. Cyanide Derivative (MW 300)
This technique enables sample analysis without the need
for a chromatograph (Fig. 1).
The DPiMS-2020, which combines PESI with a mass
spectrometer, is suitable for simple screening analysis
because it enables quick analysis of target components in
samples without pretreatment regardless of whether
samples are in liquid or solid form.
This article introduces a rapid screening method for
detecting cyanide in blood serum that does not require Analysis Sequence Without Pretreatment
pretreatment by utilizing the DPiMS-2020 and In-Source
CID.
T. Murata

Probe

Ion introduction unit (DL)

Small sample plate

DPiMS-2020