Page 8 - LifeScience Solutions for Stem Cell Analysis

A Micro-fluidic Approach using A Micro-fluidic Approach
a Super-functional Liver Chip
using a Super-functional Liver Chip
Micro-fluidicɹ Liver Hepatic

˙ Introduction
Proteomic Analysis of Human iPS Cell Differentiation Alternatives to animal testing, i.e., in vitro assay systems, are lobule

urgently required for drug, cosmetic and dietary supplement
development. The liver, a multifunctional organ, is the organ
primarily responsible for the metabolism and detoxification of
xenobiotics. Thus, the development of an artificial liver with
both iPS cells and ES cells has been investigated around the
world to replace the animal testing. Space of Disse Stellate cell
Comparative Metabolomics of Human ES Cells chip in collaboration with the Tagawa Lab, Tokyo Institute of Blood flow Hepatocyte Endothelial cell
Shimadzu also has been developing a super-functional liver

Technology, JAPAN.

Kupffer cell

The above figure shows the structure of liver. Since the hepatic tissue structure includes specific cellular polarities strongly
A Micro-fluidic Approach using a Super-functional Liver Chip ɾ Long-term maintenance of liver-specific expressions and functions Through hole Compressed Air Room Chamber
related to the multiple functions of the liver, it is important to use this architecture in the construction of an in vitro system.

While a hepatocyte chip has been widely investigated, we are developing a hepatic tissue chip, which has the following unique
features:

ɾ Flow based chip system allows control of cell culture

Metabolomics by LC-MS/MS

Diaphragm Chanel from the Cavity
incubation chamber
Chip and alignment jig Structure of the pneumatic sampling valve
Tissue Imaging by MALDI Pneumatic valve Medium

&

Drug

Filter Metabolites
PDMS
Shimadzu’s Mass Spectrometers

ECM
Hydrophobic layer

Endothelial Cells

Hepatocytes

(EHS Gel)

Structure of the super-functional liver chip
8