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Proteomic Analysis of Human   Proteomic Analysis of Human
      iPS Cell Differentiation
        MALDI-TOF MS         iPS Cell Differentiation



            ˙  Introduction
   Proteomic Analysis of Human   iPS Cell Differentiation  Induced pluripotent stem cells (iPSCs) are expected to contribute greatly to

            regenerative medicine and disease treatment. Each of the established iPSCs exhibits
            different properties, such as differentiation and proliferation capacities. However, it
            is difficult to know how the properties of iPSCs are regulated, and there are no
            markers that can discriminate the properties of each iPSC. Since the status of iPSCs
            might be regulated at various levels (e.g. genome, mRNA, protein and metabolite),
            “omics” technologies are useful in the understanding of the status of iPSCs. Among
   Comparative Metabolomics  of Human ES Cells  modification data. For example, histone modifications such as lysine acetylation,
            “omics” technologies, the proteomic approach is one of the most promising
            approaches because it gives protein level information including post translational

            lysine and arginine methylation are thought to be a deeply involved epigenetic
            mechanism for the regulation of gene expression.

            MALDI-TOF MS and LC-MS (LC-MS/MS) have been widely used in proteomics research.
            MALDI-TOF MS, combined with various separation techniques (e.g. gel electrophoresis and HPLC), have been used and are still
   A Micro-fluidic Approach using a Super-functional Liver Chip  powerful technologies because of the simple operation of MALDI-TOF MS and easily interpreted MS spectra.





                                   AXIMA Resonance / AXIMA Performance

                                                        n
               Combining the simplicity of MALDI, the power of MS , and the accuracy and resolution of TOF, the AXIMA Resonance
               provides researchers a unique solution to the next generation of structural and sequencing challenges.
               The AXIMA Performance is one of the most powerful tools in mass spectrometry, delivering information-rich spectra with
               greater sensitivity and higher confidence in identification.
   Metabolomics  by LC-MS/MS










   Tissue Imaging  by MALDI











   Shimadzu’s Mass  Spectrometers  MALDI-TOF Mass Spectrometer      MALDI-TOF Mass Spectrometer




                                                                        AXIMA Performance
                                     AXIMA Resonance





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