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Proteomic Analysis of Human Proteomic Analysis of Human
iPS Cell Differentiation
MALDI-TOF MS iPS Cell Differentiation
˙ Introduction
Proteomic Analysis of Human iPS Cell Differentiation Induced pluripotent stem cells (iPSCs) are expected to contribute greatly to
regenerative medicine and disease treatment. Each of the established iPSCs exhibits
different properties, such as differentiation and proliferation capacities. However, it
is difficult to know how the properties of iPSCs are regulated, and there are no
markers that can discriminate the properties of each iPSC. Since the status of iPSCs
might be regulated at various levels (e.g. genome, mRNA, protein and metabolite),
“omics” technologies are useful in the understanding of the status of iPSCs. Among
Comparative Metabolomics of Human ES Cells modification data. For example, histone modifications such as lysine acetylation,
“omics” technologies, the proteomic approach is one of the most promising
approaches because it gives protein level information including post translational
lysine and arginine methylation are thought to be a deeply involved epigenetic
mechanism for the regulation of gene expression.
MALDI-TOF MS and LC-MS (LC-MS/MS) have been widely used in proteomics research.
MALDI-TOF MS, combined with various separation techniques (e.g. gel electrophoresis and HPLC), have been used and are still
A Micro-fluidic Approach using a Super-functional Liver Chip powerful technologies because of the simple operation of MALDI-TOF MS and easily interpreted MS spectra.
AXIMA Resonance / AXIMA Performance
n
Combining the simplicity of MALDI, the power of MS , and the accuracy and resolution of TOF, the AXIMA Resonance
provides researchers a unique solution to the next generation of structural and sequencing challenges.
The AXIMA Performance is one of the most powerful tools in mass spectrometry, delivering information-rich spectra with
greater sensitivity and higher confidence in identification.
Metabolomics by LC-MS/MS
Tissue Imaging by MALDI
Shimadzu’s Mass Spectrometers MALDI-TOF Mass Spectrometer MALDI-TOF Mass Spectrometer
AXIMA Performance
AXIMA Resonance
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