Page 12 - LifeScience Solutions for Stem Cell Analysis

Tissue Imaging by MALDI
Tissue Imaging by MALDI
MALDI-TOF MS

˙ Introduction
Proteomic Analysis of Human iPS Cell Differentiation The use of MALDI to analyze biomolecules (e.g. low-molecular-weight metabolites,

lipids, peptides, and proteins) directly from biological tissue sections is attracting
increasing attention in research such as disease-related biomarker discovery because
spatial information of the target biomolecules is easily obtained. Generally, the
matrix solution is applied uniformly on the tissue section using a spotter (CHIP-1000)
or other such applicator. The CHIP-1000 deposits discrete droplets minimizing
diffusion with high reproducibility and maintains localization of components within
Comparative Metabolomics of Human ES Cells After deposition of the matrix, analysis is conducted on the tissue section by
the tissue. Thus, MALDI matrix is precisely delivered to specific surface locations via
a non-contact spotting method, minimizing the possibility of contamination.

MALDI-TOF MS to detect the m/z values of target biomolecules. Intensity mapping
software visualizes the spatial distribution of components of interest.

A Micro-fluidic Approach using a Super-functional Liver Chip Since biological samples have very complex structures and interconnections, the study of low molecular weight metabolites,
peptides, and proteins, between adjacent tissues can reveal important functional roles. Thus, microscopic analysis with spatial
resolution can yield novel information on the function or regulation of various tissues. As an example, analysis of spatiotemporal
changes in energy metabolism in a murine middle-cerebral artery occlusion model is presented.

MS Imaging System

CHIP-1000 - Micro-scale reagent delivery
Delivers pico-liter volumes of reagent to a
Metabolomics by LC-MS/MS tissue surface using piezo-electric technology.

Axima™ MALDI Mass Spectrometry
Sensitive and flexible MALDI mass spectrometry, ideal for imaging and profiling experiments.

Tissue Imaging by MALDI

Shimadzu’s Mass Spectrometers MS Imaging System

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