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Tissue Imaging by MALDI
MALDI-TOF MS
Data

m/z 426
[ADP-H] -

m/z 506 iPS Cell Differentiation Proteomic Analysis of Human
[ATP-H] -

m/z 346
[AMP-H ] - m/z 664 m/z 904
[NADH-H] - [Sul fatide-H] -

Fig. 1 Mass spectrum of normal brain tissue section of Human ES Cells Comparative Metabolomics
Fig. 1 shows the mass spectrum of the
control (normal) section obtained by the
mass spectrometer. In the mass spectrum
of Fig. 1, peaks generated from several
different metabolites are observed.
Metabolites associated with energy
m/z 346 metabolism that play important roles in a Super-functional Liver Chip A Micro-fluidic Approach using
AMP the body are included.

When MALDI imaging was conducted on
these metabolites, their respective
m/z 426 distribution within the brain were found
ADP to vary widely (Fig. 2).

With respect to the MALDI imaging results by LC-MS/MS Metabolomics
in the ischemic brain, a greatly reduced
m/z 506
presence of the energy metabolites such as
ATP
ADP and ATP was observed at the ischemic
site.
m/z 664 Furthermore, this tendency was more
NADH pronounced when comparing the
High
durations of occlusion, in which
differences in the presence of ADP and by MALDI Tissue Imaging
ATP were more significant at 60 minutes
m/z 904
of sustained ischemia than with the 10
Sulfatide
minute treatment.
Low
Dotted line-enclosed region: Ischemic sites
BioMap software was used for producing the mass images. By conducting MALDI imaging on a brain
ischemia mouse model, we were able to
Fig. 2 Distribution of various metabolites in murine brain tissue sections clearly distinguish between the ischemic
site and its periphery (ischemic penumbra).
Reference: K. Hattori et al. Antioxidants & Redox Signaling 13 (8) 2010, 1157-1167 Spectrometers Shimadzu’s Mass
This application is based on data obtained as a result of joint research with Dr. Makoto Suematsu, Department of Biochemistry and
Integrative Medical Biology, School of Medicine, Keio University.

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