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Comprehensive Solution for Increasing

                     Bioseparation Efficiency




                     Adsorption of biomolecules can occur not only within instruments, but also in vials, columns, and other equipment   Selecting Analytical Columns Based on Target Components and Objectives
                     used during sample preparation. Shimadzu offers support for resolving such issues with products that feature unique
                                                                                                                                        Shim-pack  Series Columns
                                                                                                                                                   ™
                     technologies for inhibiting adsorption.
                                                                                                                                        Analyzing biomolecules, such as proteins and nucleic acids, requires using columns intended for a variety of separation
                                                                                                                                        modes. In particular, samples sensitive to metal ions require a column that is optimized to reduce adsorption.
                                                                                                                                        Shimadzu offers columns optimized for a wide variety of molecules.




                                                                                                                                                                                                              Shim-pack Scepter
                                                                                                                                                                                      Reversed phase

                                                                                                                                        Nucleic Acids                                                         Shim-pack Bio IEX
                                                                                                                                        Oligonucleotides                               Ion exchange

                                                                                                                                                                                                              Shim-pack Bio Diol
                                                                                                                                                                                       Size exclusion

                                                                                                                                                                                                              Shim-pack Arata
                                                                                                                                                                                                              Shim-pack Velox
                                                                                                                                                                                      Peptide digests
                                                                                                                                                                                                              Shim-pack Bio Diol
                                                                                                                                                                                   Aggregates and Fragments
                                                                                                                                        Antibodies,
                     State-of-the-Art Technology for Inhibiting Adsorption and Increasing                                               Antibody Drug Conjugates                                              Shim-pack Bio IEX
                                                                                                                                                                                      Charge variants
                     Recovery Rates                                                                                                                                                                           Shim-pack Bio HIC
                                                                                                                                                                                       Conjugates
                     TORAST-H Bio Vials

                     Biomolecules are most likely to adsorb to vials they contact the longest. TORAST-H Bio vials offer exceptional resis-
                     tance to adsorption by biomolecules, which helps maximize recovery rates from valuable samples and increase
                     sensitivity for analyzing trace components.



                                  uV                               uV
                                 30000
                                      Basic compound A  : TORAST-H Bio Vial  4000  Basic compound B  : TORAST-H Bio Vial
                                      logP : 4.9       (100%)         logP : 0.16      (100%)
                                 20000  pKa : 9.4     : Other vender's vial A  3000  pKa : 9.6  : Other vender's vial A
                                                       (80%)                           (86%)
                                                      : Other vender's vial B         : Other vender's vial B
                                                       (38%)      2000                 (69%)
                                 10000
                                                                  1000
                                   0
                                          1.5   2.0    2.5    3.0 min  1.0  1.2  1.4  1.6  1.8  2.0  2.2  min
                                  uV                               uV
                                 30000                            50000
                                      Basic compound C  : TORAST-H Bio Vial  Basic compound D  : TORAST-H Bio Vial
                                      logP : 4.8       (100%)     40000  logP : 3.4    (100%)
                                 20000  pKa : 9.4     : Other vender's vial A  pKa : 9.1  : Other vender's vial A
                                                       (85%)      30000                (77%)
                                                      : Other vender's vial B         : Other vender's vial B
                                 10000                 (36%)                           (18%)
                                                                  20000
                                                                  10000
                                   0
                                                                    0
                                     1.0    1.5    2.0    2.5  min   1.1  1.2  1.3  1.4  1.5  1.6  1.7  min

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