Page 6 - Shimadzu Nexera XS inert

Assured Reliability and Reproducibility

Consistent Analysis Reproducibility Improves Quantitative Analysis Performance

In order to reduce the effect of adsoprtion of metal-sensitive compounds, a system is often passivated by repeatedly Adsorption of target compounds can be especially detrimental in the low-concentration region, and can worsen
injecting samples that contain the target compounds before starting data acquisition. However, this approach not calibration curve linearity. The Nexera XS inert achieves a wider dynamic range by strongly increasing the quantitative
only wastes valuable samples and time, but can make it extremely difficult to acquire reliable data due to changes in accuracy and detection of low-concentration samples.
the state of passivation during continuous analysis. Nexera XS inert systems eliminate the need for the preliminary
passivation and provide highly reliable data from the first injection and throughout the analytical session.

mAU ×10 5
7.0 Nexera XS inert + Metal-free column
First injection Tenth injection ATP
mAU mAU
250 250 125 Nexera XS inert 6.0 Standard UHPLC (stainless steel-based)
200 ATP 200 ATP + + stainless steel column
Metal-free column
100 5.0
2
Nexera XS inert 150 150 r = 0.9999
+ 4.0
Metal-free column 100 100 75 Area
50 50 Standard UHPLC 3.0
(stainless steel-based)
50
0 0 + r = 0.9918
2
0.0 0.5 1.0 1.5 2.0 min 0.0 0.5 1.0 1.5 2.0 min Stainless steel 2.0
25 column
1.0
×10 5 0 0
Nexera XS inert 1.3 1.4 1.5 1.6 1.7 min 0 10 20 30 40 g/mL
RSD% = 0.56 (n=10) Concentration
8.0

6.0
QC Sample Quantitative Analysis Results (stainless steel-based standard UHPLC)
Area Standard UHPLC
4.0 (stainless steel-based) QC Sample Spiked Conc. (µg/mL) Intra-Assay (n = 6)
RSD% = 10.1 (n=10) Measured Conc. (µg/mL) Accuracy (%) Precision (%)
Low 2 4.53 226 1.6
Medium 20 18.4 91.9 5.7
2.0
High 45 46.4 103 3.3

0
0 2 4 6 8 10
QC Sample Quantitative Analysis Results (Nexera XS inert)
Number of Injections
Intra-Assay (n = 6)
QC Sample Spiked Conc. (µg/mL)
Measured Conc. (µg/mL) Accuracy (%) Precision (%)
Low 2 2.01 101 0.78
First injection Tenth injection
mAU mAU Medium 20 20.4 102 0.35
250 250
High 45 45.0 100 0.79
200 200
Standard UHPLC 150
(stainless steel-based) 150
+ 100 100 ATP
Stainless steel column ATP
50 50
0 0
0.0 1.0 1.5 2.0 min 0.5 1.0 1.5 2.0 min

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