Page 4 - Shimadzu Nexera XS inert

Unconstrained Recovery and Sensitivity

For the quantitative analysis of biomolecules, particularly when high sensitivity is required, the adsorption of the target
compound to a metal surface can result in a dramatic decrease in sensitivity. The Nexera XS inert system prevents peak
tailing and sensitivity loss by eliminating adsorption of proteins, nucleic acids, and other components to metal surfaces.
As a result, reliable and accurate quantitation is possible, even for low-concentration samples.

2 ng 100 ng
mAU mAU
200 200
ADP AMP ADP
AMP
ATP ATP
150 150
Nexera XS inert
+ 100 100
Metal-free column
50 50

0 0
0.0 2.5 min 0.0 2.5 min

mAU mAU
200 200
AMP
AMP
150 150
Standard UHPLC
(stainless steel-based) 100 100 ADP
+
Stainless steel column ATP
50 50
Clear Resolution without Restrictions
0 0
0.0 2.5 min 0.0 2.5 min

The Nexera XS inert system is equipped with unique technology that ensures the complete inertness of the sample
The Nexera XS inert can detect oligonucleic acids with a strong tendency of metal adsorption. flow path. The system provides excellent peak shape and unsurpassed chromatographic separation by effectively
inhibiting the adsorption of target compounds to internal surfaces.

×10 3
10.0
Negative Nexera XS inert Standard UHPLC (stainless steel-based) Nexera XS inert
m/z 803.5>95.00 Standard UHPLC mAU mAU
8.0 (stainless steel-based) AMP ADP AMP
30 30
×1.7 20 ATP
6.0 20 ADP
Intensity (cps) 4.0 10 10 0

0
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 min 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 min
2.0
Metal-sensitive Material that inhibits
Poor peak shape Sharp peaks
compounds adsorption
Adsorption to internal surface Peak tailing Excellent separation
0
Stainless steel tubing
5.75 6.00 6.25 6.50 min

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