Page 5 - Shimadzu Nexera XS inert
P. 5

Unconstrained Recovery and Sensitivity







 For the quantitative analysis of biomolecules, particularly when high sensitivity is required, the adsorption of the target
 compound to a metal surface can result in a dramatic decrease in sensitivity. The Nexera XS inert system prevents peak
 tailing and sensitivity loss by eliminating adsorption of proteins, nucleic acids, and other components to metal surfaces.
 As a result, reliable and accurate quantitation is possible, even for low-concentration samples.


 2 ng  100 ng
 mAU  mAU
 200  200
 ADP  AMP  ADP
 AMP
 ATP  ATP
 150  150
 Nexera XS inert
 +  100  100
 Metal-free column
 50  50

 0  0
 0.0  2.5  min  0.0  2.5  min

 mAU  mAU
 200  200
 AMP
 AMP
 150  150
 Standard UHPLC
 (stainless steel-based)  100  100  ADP
 +
 Stainless steel column  ATP
 50  50
               Clear Resolution without Restrictions
 0  0
 0.0  2.5  min  0.0  2.5  min



               The Nexera XS inert system is equipped with unique technology that ensures the complete inertness of the sample
 The Nexera XS inert can detect oligonucleic acids with a strong tendency of metal adsorption.  flow path. The system provides excellent peak shape and unsurpassed chromatographic separation by effectively
               inhibiting the adsorption of target compounds to internal surfaces.

 ×10 3
 10.0
 Negative  Nexera XS inert  Standard UHPLC (stainless steel-based)             Nexera XS inert
 m/z 803.5>95.00  Standard UHPLC  mAU                           mAU
 8.0  (stainless steel-based)        AMP                               ADP    AMP
                    30                                         30
 ×1.7                                                          20      ATP
 6.0                20        ADP
 Intensity (cps)  4.0  10                                      10 0

                    0
                     0.0  1.0  2.0  3.0  4.0  5.0  6.0  7.0  min  0.0  1.0  2.0  3.0  4.0  5.0  6.0  7.0  min
 2.0
               Metal-sensitive                                 Material that inhibits
                                                 Poor peak shape                           Sharp peaks
               compounds                                       adsorption
                         Adsorption to internal surface  Peak tailing                      Excellent separation
 0
               Stainless steel tubing
 5.75  6.00  6.25  6.50  min


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