Page 11 - Shimadzu Nexera XS inert

Comprehensive Solution for Increasing

Bioseparation Efficiency

Adsorption of biomolecules can occur not only within instruments, but also in vials, columns, and other equipment Selecting Analytical Columns Based on Target Components and Objectives
used during sample preparation. Shimadzu offers support for resolving such issues with products that feature unique
Shim-pack Series Columns
™
technologies for inhibiting adsorption.
Analyzing biomolecules, such as proteins and nucleic acids, requires using columns intended for a variety of separation
modes. In particular, samples sensitive to metal ions require a column that is optimized to reduce adsorption.
Shimadzu offers columns optimized for a wide variety of molecules.

Shim-pack Scepter
Reversed phase

Nucleic Acids Shim-pack Bio IEX
Oligonucleotides Ion exchange

Shim-pack Bio Diol
Size exclusion

Shim-pack Arata
Shim-pack Velox
Peptide digests
Shim-pack Bio Diol
Aggregates and Fragments
Antibodies,
State-of-the-Art Technology for Inhibiting Adsorption and Increasing Antibody Drug Conjugates Shim-pack Bio IEX
Charge variants
Recovery Rates Shim-pack Bio HIC
Conjugates
TORAST-H Bio Vials

Biomolecules are most likely to adsorb to vials they contact the longest. TORAST-H Bio vials offer exceptional resis-
tance to adsorption by biomolecules, which helps maximize recovery rates from valuable samples and increase
sensitivity for analyzing trace components.

uV uV
30000
Basic compound A : TORAST-H Bio Vial 4000 Basic compound B : TORAST-H Bio Vial
logP : 4.9 (100%) logP : 0.16 (100%)
20000 pKa : 9.4 : Other vender's vial A 3000 pKa : 9.6 : Other vender's vial A
(80%) (86%)
: Other vender's vial B : Other vender's vial B
(38%) 2000 (69%)
10000
1000
0
1.5 2.0 2.5 3.0 min 1.0 1.2 1.4 1.6 1.8 2.0 2.2 min
uV uV
30000 50000
Basic compound C : TORAST-H Bio Vial Basic compound D : TORAST-H Bio Vial
logP : 4.8 (100%) 40000 logP : 3.4 (100%)
20000 pKa : 9.4 : Other vender's vial A pKa : 9.1 : Other vender's vial A
(85%) 30000 (77%)
: Other vender's vial B : Other vender's vial B
10000 (36%) (18%)
20000
10000
0
0
1.0 1.5 2.0 2.5 min 1.1 1.2 1.3 1.4 1.5 1.6 1.7 min

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