Page 57 - Application Notebook - Solution for Food Safety

Application No.L512
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Table 2 Analytical Conditions 4BNQMF
System : Nexera-i 3D, RF-20AXS
Column : Shim-pack GIST C18 (75 mm L. × 3.0 mm I.D., 2 μm) &YUSBDUJPO
Mobile Phase : A) 20 mmol/L (Sodium) phosphate buffer (pH 2.5) 'JMUFSJOH
B) Acetonitrile

C) Methanol .ZDP4QJOä .VMUJ4FQš
(Gradient elution) $MFBOVQ DPMVNO
Flowrate : 1.0 mL/min
Column Temp. : 55 ˚C
Injection Vol. : 10 μL $MFBOVQ
Detection AFB1, AFB2, AFG1, AFG2, AFM1
(RF-20AXS) : : Ex 365 nm, Em 450 nm
OTA, ZON : Ex 320 nm, Em 465 nm *OKFDU UP )1-$
Detection : NIV, DON : 220 nm (ch 1)
(Nexera-i 3D) PAT : 276 nm (ch 2) Fig. 4 Sample Pretreatment
Although regulatory limits for mycotoxin levels in food 3 mV ˙Peaks: See Fig. 3.
can vary by country and region, the screening system is RF-20AXS 2 4 A: Standard mixture
compatible with the strictest regulatory limits that are 2 B: Flour
C: Flour [spiked]
found in the EU (excluding regulatory limits in baby
food). Chromatograms of a standard mixture with
1)
mycotoxin levels equivalent to EU reference levels is 1 5 7
shown in Fig. 3. 3 6 A
0 B
mV C
3
RF-20AXS 4 ˙Peaks
1) AFM1 1 μg/L 4 5 6 7 8 9 10 11
2 2) AFG2 0.25 μg/L min
2 3) AFG1 0.25 μg/L mAU
1 4) AFB2 0.25 μg/L Nexera-i PDA
5) AFB1 0.25 μg/L 8 9
6) ZON 50 μg/L 5
1
7) OTA 5 μg/L
7
5 A
3 6
0
0 B
C
4 5 6 7 8 9 10 11
min 0.0 0.5 1.0 1.5 2.0 2.5 3.0
mAU min
Nexera-i PDA ˙Peaks
5 8) NIV 1000 μg/L Fig. 5 Chromatograms of Soft Wheat Flour
8 9) DON 1000 μg/L
10) PAT 25 μg/L
9 mV ˙Peaks: See Fig. 3.
3
RF-20AXS 4 A: Standard mixture
0 220 nm 2
10 B: Rice flour
276 nm 2 C: Rice flour [spiked]
0.0 0.5 1.0 1.5 2.0 2.5 3.0
min 1 7
5
3 6
Fig. 3 Chromatograms of a Standard Mixture A
0 B
Q Analysis of Mycotoxins in Grain C
This section describes an analysis of milled grains after 4 5 6 7 8 9 10 min
11
pretreatment. Fig. 4 shows an overview of the sample mAU
pretreatment method. Further details can be found in Nexera-i PDA
the mycotoxin screening system instruction manual. 5 8 9
Chromatograms of pretreated samples of soft wheat
flour and rice flour and of pretreated samples of soft A
wheat flour and rice flour spiked with a standard 0 B
mixture of mycotoxins that are produced in grains C
(shown among the screening target compounds listed
in Table 1) are shown in Fig. 5 and Fig. 6. 0.0 0.5 1.0 1.5 2.0 2.5 3.0
Comparing the area of each peak in the standard min
mixture that contains mycotoxins at EU reference levels Fig. 6 Chromatograms of Rice Flour
and each peak in the flour samples allows identification
of whether the mycotoxins present in flour samples are Footnotes
1) Converted concentrations in the standard mixture were obtained
in excess and violation of reference levels. The system according to a pretreatment method described in the i-Series
makes this determination without the need for complex Solution Package Mycotoxin Screening System instruction manual.
analysis of results by the user, allowing for easy 2) MycoSpinä 400 and MultiSepš 227 are registered trademarks of
screening of target compounds. Romer Labs.
First Edition: Sep. 2016
For Research Use Only. Not for use in diagnostic procedure.
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