Page 56 - Application Notebook - Solution for Food Safety

LAAN-A-LC-E288

Application High Performance Liquid Chromatography

News Analysis of Mycotoxins in Grain Using Mycotoxin

Screening System
No.L512

Mycotoxins are chemical products produced by Q Analysis of a Standard Solution
organisms in the fungus kingdom and are toxic to Mycotoxin targets of the screening system are shown in
humans, animals, and crops. As an example, aflatoxins Table 1, chemical structures of some of these targets
are a type of mycotoxin that are some of the most are shown in Fig. 2, and analytical conditions are shown
carcinogenic naturally occurring substances in the in Table 2. The package includes analysis method files
world. They are classified as Group 1 carcinogens that eliminate the need to configure analytical
(carcinogenic to humans) by the WHO International conditions. An RF-20AXS was used to perform analysis
Agency for Research on Cancer (IARC), and subject to with on-time excitation wavelength/emission
strict regulations in many countries and regions of the wavelength switching.
world.
This Application News describes the screening analysis Table 1 Screening Target Compounds
for mycotoxins in grain products (soft wheat flour and Mycotoxin Abbreviation Matrix
rice flour) using the i-Series Solution Package mycotoxin 1
screening system. Aflatoxin M1 AFM1 Milk
2 Aflatoxin G2 AFG2

Q i-Series Solution Package Mycotoxin Screening 3 Aflatoxin G1 AFG1

System 4 Aflatoxin B2 AFB2

The screening system comprises a compact and easy to 5 Aflatoxin B1 AFB1 Grain
use integrated i-Series HPLC system together with 6 Zearalenone ZON
analysis methods including sample pretreatment 7 Ochratoxin A OTA
methods. The system comes ready to use and capable 8 Nivalenol NIV
of data acquisition and analysis, including columns and 9 Deoxynivalenol DON
method files designed for mycotoxin analysis, an 10 Patulin PAT Apple
instruction manual with analysis methods, and report
templates. For screening applications, the system can
determine whether mycotoxin levels in food are in AFB1 AFB2
excess of reference levels.

Column AFG1 AFG2

AFM1 ZON
CD-ROM
Mixer
Nexera-i 3D, RF-20AXS

Fig. 1 Mycotoxin Screening System
OTA PAT
Currently, HPLC and LC/MS are the most common
techniques used to identify aflatoxins in food. With
HPLC, fluorescent derivatization is often performed to
improve sensitivity, though disadvantages of
derivatization procedures are the time required and
their complexity. Meanwhile, though LC/MS is more NIV DON
selective in terms of sensitivity, major financial
investment into system is required.
The i-Series Solution Package comes with a built-in PDA
detector, and can be further enhanced with an RF-
20AXS fluorescence detector that offers world-class
Fig. 2 Target Mycotoxin Structures
sensitivity. The package can also detect aflatoxins
directly without derivatization.