Page 48 - Application Notebook - Solution for Food Development
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Application  No.C132
    News

            n Comparison of Sugars (Flesh and Peel)            n Comparison of Citrus Fruits (Flesh)
            The results of a quantitative comparison of sugars in   Mikan orange and its hybrid varieties tended to appear
            flesh and peel are shown in Fig. 6. Although there is no   on the upper right of the score plot. Since we confirmed
            difference in the total quantity of sugars present in   that carotenoids appear in the upper right of the loading
            flesh and peel, the sugar composition of flesh and peel   plot (Fig. 8), we also performed a quantitative analysis of
            differs substantially.                             carotenoids. This quantitative analysis confirmed mikan
                                                               orange and its hybrid varieties have a high carotenoid
                                                               content, and in particular contain a high quantity of
             (mg/g)  Amount (mean)       Composition           β-cryptoxanthin and β-carotene (Fig. 9).
              150                            Sucrose
              100                      Fructose
                                Sucrose            Fructose                       Score plot
              50                            Glucose
                                       Glucose
               0
                   Flesh  Peel        Flesh      Peel               Hybrid of mikan orange    Mikan orange
                  Fig. 6  Comparison of Sugar Quantity and Sugar
                       Composition in Flesh and Peel



            n Comparison of Secondary Metabolites (Flesh and Peel)
            The results of a quantitative comparison of secondary                         R2 (PC1): 30 %
            metabolites in flesh and peel are shown in Fig. 7. Flesh                      R2 (PC2): 23 %
                                                                                          Scaling: Unit variance
            contained ≥ 30 times the quantity of carotenoids
            compared to peel. Meanwhile, peel contained around
            five times the quantity of flavonoids compared to flesh.
            These results showed a substantial difference in the
            secondary metabolites of flesh and peel.                            Loading plot

                     $BSPUFOPJET             'MBWPOPJET                                        Carotenoids
              OH H                     NH H




                    'MFTI  1FFM             'MFTI   1FFM
                 Fig. 7  Comparison of Mean Secondary Metabolites

                                                                    Fig. 8  Principal Component Analysis (PCA) of Flesh



                                                                (ng/g)          Ќ-carotene
                                                                5000                                    VIO
                                                                4000               Ќ-cryptoxanthin      LUT
                                                                                                        ZEA
                                                                3000
                                                                                                        BCA
                                                                2000
                                                                                                        BCR
                                                                1000
                                                                   0




                                                                Fig. 9  Comparison of Carotenoid Content of Seven Citrus Fruits



                                                                                                      First Edition: Jul. 2016
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