Page 47 - Application Notebook - Solution for Food Development

Application No.C132
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n Multiple Reaction Monitoring (MRM) Analysis of Primary and Secondary Metabolites
The MRM chromatograms obtained for compounds analyzed under each set of analytical conditions are shown in
Fig. 4. The calibration curve ranges for all compounds are also shown in Table 2.

Table 2 Calibration Curve Ranges of Target Compounds
(×100,000)
2.5 Conditions 1 Tangeretin Amino acids Range Organic acids Range
organic acid
2.0 amino acid Cystine 1-100 Tartaric acid 50-10000
Flavonoid
1.5 Nobiletin Aspartic acid 5-100 2-Ketoglutaric acid 10-1000
Asparagine 5-100 Isocitric acid 50-10000
1.0 Kaempherol
Quercetin Serine 5-100 Malic acid 10-5000
0.5 4-Hydroxyproline 1-100 Lactic acid 50-10000
Naringin
0.0 Glycine 5-100 Citric acid 50-10000
0.0 2.5 5.0 7.5 10.0 12.5 min Lysine 1-100 Pyroglutamic acid 10-10000
(×1,000,000)
1.50 Conditions 2 Cysteine 50-100 Succinic acid 10-1000
β-carotene Threonine 5-100 Fumaric acid 500-1000
1.25
Fucoxanthin Canthaxanthin Glutamic acid 1-100 Maleic acid 50-10000
1.00 Lycopene
Alanine 5-100
0.75 Proline 1-100
Lutein β-cryptoxanthin
0.50 Ornitine 5-1000
Violaxanthin
0.25 Zeaxanthin Glutamine 5-100
Astaxanthin Histidine 5-100
0.00
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 min Arginine 5-100
(×1,000,000) GABA 5-100
Conditions 3
1.25 Glucose Valine 1-100
Methionine 5-100
1.00 Mannose
Tyrosine 5-100
0.75 Fructose Isoleucine 5-100
Rhamnose
0.50 Leucine 10-100
Maltose, Lactose
0.25 Sucrose Phenylalanine 1-100
Tryptophan 5-500
0.00
2.5 5.0 7.5 10.0 12.5 min
Sugars Range
Fig. 4 Example Chromatograms Obtained Under Rhamnose 50-1000
Three Analytical Conditions Fluctose 50-5000
Glucose 50-5000
Sucrose 100-5000
Maltose, Lactose 200-2000
Carotenoids Range Flavonoids Range
n Comparison of Flesh and Peel Constituents Fucoxanthin 0.1-100 Naringin 10-1000
Constituents of the flesh and peel of the seven citrus Violaxanthin 1-100 Quercetin 5-1000
fruits were compared by performing principal Astaxanthin 0.5-100 Kaempferol 5-1000
component analysis. Flesh and peel constituents tended Lutein 0.1-100 Nobiletin 0.1-1000
0.5-100 Tangeretin
to separate into two groups on a score plot. The results Zeaxanthin 0.05-100 0.1-1000
Canthaxanthin
of the loading plot indicate that the carotenoids, Ќ-Cryptoxanthin 0.05-100
flavonoids, and sugars content of flesh and peel Lycopene 50-100
separates these sample types. Ќ-carotene 0.1-100 (µg/L)
Score plot Loading plot
Peel Flesh
Carotenoids

Sugars

Flavonoids
Sugars
R2 (PC1) 30 %
R2 (PC2) 17 %
Scaling Unit variance

Fig. 5 Principal Component Analysis (PCA) of Flesh and Peel