Page 43 - LC-SFC_Pharma_Brochure

3. Summary of i-PDeA Settings 4. Conclusion

The parameters for the i-PDeA function are set as part of the data The excellent performance and reproducibility of the SPD-M30A
processing method of the photodiode array detector. Once the photodiode array detector and the Nexera X2 system make this
analytical protocol is defined, it can be applied for routine analy- new separation methodology possible. The i-PDeA function helps
sis. The following is a brief summary of the i-PDeA parameters. increase the speed of analysis and enhances laboratory produc-
tivity. To summarize the key benefits:
1. The Savitzky-Golay method is used to determine the first deriv-
Co-eluted peaks can be separated mathematically, using deriv-
ative spectrum, from which a list of wavelengths where the 1 st
ative spectrum chromatograms
derivative value is zero is generated. Spline interpolation is ap-
st
plied to calculate the wavelength closest to the 1 derivative Poorly resolved peaks are processed and visualized as pure
zero wavelengths, and the results are displayed in a table. peaks with no contribution from co-eluting components
Impurity peaks hidden by, or even in, the target peak can be
detected
Fast and accurate quantitative analysis is possible, even without
complete chromatographic separation
Simple post-run analysis procedure
i-PDeA most effectively resolves co-eluted peaks when the peak
The i-PDeA function provides a new solution, which is useful for
height in the derivative spectrum chromatogram for one com-
identification and quantitation of impurities. The use of this fea-
st
ponent, taken at a 1 derivative zero wavelength of another
ture is expected to increase laboratory efficiency and produce
component, is sufficiently large. If the shape of the spectra of
more reliable analytical data.
two components is very similar, i-PDeA cannot be applied.
2. To extract the derivative spectrum chromatogram, plotting the
derivative spectrum values at the specified wavelength against
References
retention time, select “Derivative” for Chromatogram Type
Anal. Chim. Acta
1) A. Lober, , 164, 293-297 (1984)
and using the wavelength obtained by the Detect 1 Derivative
st
2) B. Kowalski ., ., 58, 496-499 (1986)
et al
Anal. Chem
Zero function, set the value (with 2 decimal places) of the
et al
3) T. Ryan ., ., 16 (7) , 1545-1560 (1993)
J. Liq. Chromatogr
Wavelength in the Wavelength Settings window of the Multi-
Anal. Chim., Acta
4) B. Vandeginse ., , 173, 153-164 (1985)
et al
Chromatogram table.
5) M. Maeder ., ., 3, 205-213 (1988)
et al Chemom. Intell. Lab. Sys
6) I. Sakuma ., ., 506, 223-243 (1990)
et al
J. Of Chromatog
Analyst
et al
ղ૾౓ෆ଍ 7) A. Yamamoto ., , 120, 377-380 (1995)
et al ANALYTICAL SCIENCE
8) T. Hakuta ., , 25, 1149-1153 (2009)
J. Sep. Sci
9) K. Uchiyama ., ., 34, 1525-1530 (2011)
et al
10) S.Kazuhara, Food and Agricultural Materials Inspection
Center Research Report, 23, 77-86 (1999)
et al BUNSEKI KAGAKU
11) K. Uchiyama ., , 60 (2) , 171-174 (2011)
et al BUNSEKI KAGAKU
12) S. Kodama ., , 45 (3) , 259-263 (1996)
The derivative spectrum chromatogram has positive value
when the slope of the spectrum is up and negative value when
the slope of the spectrum is down. Peak direction in the deriv-
ative spectrum chromatogram can be adjusted by setting the
polarity, and peak size can be adjusted by setting the factor.
3. Set integration and quantitation parameters for the derivative
spectrum chromatogram.
The derivative spectrum chromatogram can be handled the
same as other multi-chromatograms for quantitative purposes.
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