Page 21 - Clinical-Medical Solution for Clinical Chemistry

High-sensitivity and simultaneous analysis of Psychoactive drugs
using LC-MS/MS with full-automated pretreatment system

Introduction

LC-MS/MS has become a preferred method for the routine also increasing to investigate the cause of death. However,
analysis for forensic toxicology. LC-MS/MS allows for the manual sample preparation often involves several
simultaneous analysis of multiple compounds in a single complicated manual steps which can introduce error into
run, thus enabling a fast and high throughput analysis. In the results. In this study, we investigated the processing
recent years that it seems the number of incident and capability to analyze serum, whole blood and urine spiked
accident is increasing caused by dosed with psychotropic sixty psychotropic drugs by LC-MS/MS with automated
drugs and the number of drug testing with LC-MS/MS is sample preparation unit.

Group 1. Eight Barbiturate drug and Bromovalerylurea

Allobarbital Amobarbital Barbital Pentobarbital Phenobarbital
Secobarbital Thiamylal Thiopental Bromovalerylurea

Group 2. twelve Tri-/Tetra-cyclic antidepressant

Amitriptyline Amoxapine Clomipramine Desipramine Dosulepin Imipramine
Maprotiline Mianserin Nortriptyline Promethazine Setiptiline

Group 3. Thirty-nine Benzodiazepines and their metabolites

Alprazolam Bromazepam Brotizolam Chlordiazepoxide Clorazepic

acid Clotiazepam Cloxazolam Diazepam Estazolam Ethyl
lo azepate Etizolam Fludiazepam Flunitrazepam Flurazepam

Flutazolam Flutoprazepam Haloxazolam Lorazepam Lormetazepam

Medazepam Mexazolam Midazolam Nimetazepam Nitrazepam
Oxazolam Prazepam

Quazepam Rilmazafone To sopam Triazolam Zolpidem

7-Amino unitrazepam 7-Aminonimetazepam 7-Aminonitrazepam

α-Hydroxyetizolam (M-VI) α-Hydroxyalprazolam α-Hydroxybrotizolam
α-Hydroxytriazolam Zolpidem M-1

Figure 1 Target drugs

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