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High-sensitivity and simultaneous analysis of Psychoactive drugs
using LC-MS/MS with full-automated pretreatment system
Methods and Materials
The analysis of 60 psychoactive drugs (eight Barbiturate Samples were trapped on Imtakt Unison UK-C18
drug, thirty-nine Benzodiazepines and their metabolites, (10x2mm, 3.0 m), then separated by Imtakt Unison
twelve Tri-/Tetra- cyclic antidepressant and UK-C18 (75x2mm, 3.0 m) with a binary gradient system.
bromovalerylurea) were performed using a fully automatic Water with ammonium formate and methanol were used
LCMS preparation unit (CLAM-2000, Shimadzu) online for mobile phases.
with HPLC-LCMS (NexeraX2-LCMS-8060, Shimadzu).
Report creation
MS/MS
Library
-Serum search
-Whole Blood
-Urine
Figure 2 CLAM-2000 and LCMS-8060 system
Sample
Dispensing Reagent Shaking
• 50 µL Dispensing • 30sec
of serum or whole blood • 50 µL of Water
Filtration
Reagent (PTFE,
Dispensing Shaking
• 300 µL of MeOH • 60sec 0.45 m)
• 90sec
To AutoSampler
Figure 3 Analytical ow of serum and whole blood
Sample Filtration
Reagent
Dispensing Dispensing Shaking (PTFE,
• 100 µL • 100 µL of MeOH • 60sec 0.45 m)
of urine • 90sec
Figure 4 Analytical ow of urine To AutoSampler
3
