Page 19 - Clinical-Medical Solution for Clinical Chemistry

Evaluation of Blood Lysis Procedures prior to Automated
Sample Preparation for Immunosuppressant Assay by LC-MS/MS

Conclusion

• Freeze/thaw with -80ºC was ef cient for blood lysis and recovery of ISP whatever the volume.
• Freezing time was not suf cient for -20ºC in a volume of 1.0 mL or 0.2 mL.
• Ammonium chloride can be used as alternative. However, it induce sample dilution.
Thus, this should be taken into account.
• ISP recovery is correlated with lysis ef ciency.

Reference

1) Lugia Rossi, et al., Advanced Drug Delivery Reviews, 2016, 106, 73-87

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diagnostic procedures.

First Edition: June, 2017

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