Page 28 - Shimadzu-MassSpectrometerCatalog

Food ingredients alalysis

LCMS-8040 / LCMS-8030

Analysis of Geniposidic Acid and Chlorogenic Acid in Tochu Tea

Tochu tea, made from the leaves of tochu (Eucommia ulmoides), is one of the five most popular Chinese herbal medicines. Tochu tea
is rich in the iridoids geniposidic acid and chlorogenic acid (3-caffeoylquinic acid), and the antihypertensive effects of geniposidic acid
is well known. LC/MS has been employed in recent years to analyze the polyphenols in these plant extracts. MRM chromatograms of
geniposidic acid and chlorogenic acid in the 1,000 to 1 diluted tochu tea is shown (Fig. 1). Analysis was accomplished without signifi-
cant interference from impurity components. The 1,000 to 1 dilution of tochu tea contained approximately 600 to 700 ppb genipo-
sidic acid and chlorogenic acid.

373.00>123.30(-) 30000
2000 353.00>191.20(-)
A 27500 B
1750 Geniposidic acid/3.451 25000 Chlorogenic acid/7.622
1500 22500
1250 20000
17500
1000 15000
12500
750
10000
500 7500
5000
250
2500
0 0
2.0 3.0 4.0 min 6.0 7.0 8.0 9.0 min
MRM Chromatograms of Geniposidic Acid (A) and Chlorogenic Acid (B) in Tochu Tea
LCMS-8040 / LCMS-8030

Analysis of Water Soluble Vitamins
On August 24, 2007, the U.S. Food and Drug Administration (FDA) published cGMP (Current Good Manufacturing Practice) in Manu-
facturing, Packaging, Labeling, or Holding Operations for Dietary Supplements, 21 CFR (Code of Federal Regulations) Part 111. This
regulation mandates testing the ingredients in supplements. The following shows an example of using LC/MS/MS to quantitatively
analyze water soluble vitamins in a commercial dietary supplement. Nine water soluble vitamins were measured with good separation
and sensitivity. In addition, each ingredient was successfully quantitated in a supplement extract solution without being affected by
contaminant components. Quantitation results are shown below.
Thiamin
1:265.10>122.10(+)(0.80) Thiamin Pyridoxine
2:170.10>152.20(+)(0.50) Thiamin Pyridoxine
3:124.20>80.0(+)(5.00) Area (x100,000) Area (x100,000)
4:123.10>80.0(+)(0.60) Area (x100,000) Area (x1,000,000)
5:220.10>90.00(+)(1.60)
Pyridoxine 11.0 mg
6:678.55>146.80(+)(9.00) 7.5 0.5−500 ng/mL 1−500 ng/mL 1.5 15.6 mg 2.0
7:377.20>242.80(+)(10.00)
2000000 8:245.10>227.10(+)(0.80) R 2 = 0.9999 1.00 R 2 = 0.9993
Nicotinic acid 9:440.20>295.10(+)(10.00)
1.0
5.0 0.75
1.0
0.50
Nicotinamide 0.5
1500000 2.5
0.25
Pantothenic acid
0 0
0.0 0.00 1.50 2.00 2.50 min
Cyanocobalamin 0 250 Conc. 0 250 Conc. 2.50 3.00 3.50 min
1000000 Nicotinamide Compound Quantitation Results (for about 10 tablets)
Cyanocobalamin Riboflavin Area (x100,000)
Area (x100,000) Area (x10,000) Thiamin 15.6 mg
Riboflavin Pyridoxine 11.0 mg
Biotin 50−10,000 ng/mL 10−1,000 ng/mL 4.0 43.4 mg
4.0 R 2 = 0.9992 2.0 R 2 = 0.9999 Nicotinic acid 0 mg
3.0 Nicotinamide 43.4 mg
500000 1.5
Folic acid 3.0 Pantothenic acid 20.6 mg
2.0
2.0 1.0 Cyanocobalamin 23.3 µg
1.0 Riboflavin 6 mg
1.0 0.5 Biotin 35.6 µg
0 0 Folic acid 218.4 µg
0.0 0.0
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 min 0 4.00 5.00 min
0 5000 Conc. 500 Conc.
MRM Chromatograms Calibration Curves MRM Chromatograms and Quantitation Results for
of Water Soluble Vitamins of Water Soluble Vitamins Commercial Supplement Extract Solution
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