Page 30 - Shimadzu Journal vol.9 Issue2

Shimadzu

Selection These article were selected by Shimadzu. Relating Biopharmaceutical analysis and
development, they are from posters presented at ASMS 2021. They feature a variety
of instruments we produce and include cutting-edge technologies. Please obtain the
articles of your interest through the links on the titles.

Guide to Biopharmaceutical Solutions
This Biopharmaceutical Solutions Guide introduces the best solutions for each step of the biopharmaceutical development process, including cell line optimization,
culture, purification, characterization, quality control, and pharmacokinetics.

Analysis of Protein Drugs Aggregation Oligonucleotides analysis by Ion Exchange
Using Size Exclusion Chromatography Chromatography and Effects of pH Changes
This article describes an aggregate analysis using a Shimadzu in the Mobile Phase on Separation
“Shim-pack™ Bio Diol” , size exclusion chromatography column with In this article, we introduce an analytical method for the separation of
“Nexera XS inert”, an ultra high performance liquid chromatograph. oligonucleotides of different length by ion-exchange chromatography,
This chromatograph has high salt tolerance and metal-free flow path and assuming that shorter length components are impurities derived from
allows to use highly salted mobile phases and prevents sample adsorption. the synthesis process. In order to achieve optimal analytical performances
an inert UHPLC system was used. The Nexera XS inert, which is designed
to suppress the adsorption of metal-coordinating compounds containing
phosphate groups. We also report the effect of changesin mobile phase
pH on analytical results.

Achieving Improved Sensitivity and Reliable Charge variant analysis of mAb biotherapeutics by
Analytical Performances in Nucleotides Analysis Nexera XS with a Shim-pack Bio IEX Column
Stainless steel is commonly used in HPLC due to its pressure proof and Monoclonal antibodies (mAbs) are the most approved biopharmaceuticals
corrosion resistant. However, it can interact with compounds containing used to treat severe and chronic diseases such as cancer, autoimmune, car-
phosphate group(s) by metallic affinity. This is a factor that negatively diovascular, respiratory, hematology, and several infections. They have an
affecting the shape and intensity of the peak. In order to suppress metal enormous therapeutic and commercial value which makes them among
adsorption, cleaning flow path with phosphoric acid, addition of chelating the top 10 best sellers of pharmaceuticals for several past years. In recent
agents to mobile phase, or repeated injections of the target compounds years, the use of mAbs has been expanded due to significant advances
are often performed. Nevertheless, it is not easy to obtain highly repro- in design thus decreasing immunogenicity in humans, improving their
ducible results. This article introduces the use of the Nexera XS inert bioavailability, and specific affinity for antigen-binding.
ultra-high performance liquid chromatograph, which utilizes a metal-free
flow path, and a metal-free column for the accurate and reproducible
analysis of nucleotides.

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