Page 27 - Shimadzu Prominence

Evaporative Light-Scattering Detector

ELSD-LT ll

Highly Versatile Detection Method

Not all compounds have a chromophore or other such structural property that allows the use of an
absorbance detector. Refractive Index Detection (RID) is one option but it suffers from the inability to
run gradient analysis. Evaporative Light Scattering Detection (ELSD) is a perfect alternative to RID as it
is more rugged, quicker to stabilize, and gradient compatible. ELSD is ideal for applications like testing
the purity of compounds, measuring the molecular weight distribution of synthetic polymers, and
analyzing natural substances.

Analytical Conditions
Column: Shim-pack VP-ODS Detects Most Compounds
(150 mmL. × 4.6 mmI.D.)
Mobile phase: Water/Methanol Gradient
(60/40-40/60, 2%/min)
Flow rate: 1 mL/min
Temperature: 40°C
Sample: PEG-1000 With the exception of some highly volatile compounds, the ELSD-LT II is able to detect almost any
compound. Unlike traditional absorbance detectors (UV-Vis, PDA, etc.), sensitivity is not
dependent on the physical or structural properties of the compound, but rather the absolute
quantity of the solute passing through the detector cell. Therefore, it is especially useful for
Chromatogram of Non-Chromophoric Compound detecting unknown or breakdown compounds and/or validating purity of a target compound. By
this mechanism of detection the ELSD-LT II is truly a universal detector.
Photomultiplier
High Sensitivity by Low Temperature Evaporation
Light Source
The ELSD-LT II detector uses a unique nebulizer and drift tube design to achieve stable and
low-temperature evaporation of mobile phases, making it possible to analyze semi-volatile and /
or thermally unstable compounds.
Assist Gas Assist Gas
High-sensitivity detection is achieved by focusing the sample at the detection point with assist gas
Assist Gas Functions flow. The ELSD-LT II offers high sensitivity with this low-temperature evaporation technology and
superb detection technology.
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Automated Functions

Auto-Powerdown functions for the LED light source and nebulizer gas reduce operating costs. The
self-cleaning design makes maintenance of the drift tube easier.

Example of analyzing 4 semi-volatile alkyl parabens,
considered difficult to analyze with conventional ELSD detectors.
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Specifications
ELSD-LT II (228-45115-xx)
Nebulizing Method Siphon Splitting
Light source LED
Detection Photomultiplier Tube
*1: Requires gas supply source, such as a gas line, nitrogen generator, or air
Temperature setting range Ambient to 80°C compressor.
Nebulizer gas Nitrogen (N2) or Air *1 Note
Gas flow rate, Gas pressure Max. 3.0 L/min, Max. 450 kPa *An optional pressure regulator with filter is required to remove microscopic materials
Mobile phase flow rate 0.2 to 2.5 mL/min in gas.
Analog output 0 to 1 V *When using a nitrogen generator or an air compressor, please be careful that
moisture, oil, dust, etc. should not be contained in nitrogen or air.
Operating temperature range 5 to 40°C
*Please use it in the room where exhaust equipment is available.
Max. 80% (5 to 31°C, room temperature)
Operating humidity range
Max. 50% (31 to 40°C, room temperature)
Size, Weight W250 × D550 × H450 mm, 20 kg
Power supply AC 115 V, 230 V, 150 VA, 50/60 Hz

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High Performance Liquid Chromatograph