Page 9 - Shimadzu Nexera X2

Broad Injection Volume Range Enables Both UHPLC and HPLC

Nexera X2 series autosamplers offer 2,500,000
R = 0.999795
2
excellent injection reproducibility over a
2,000,000
wide range of injection volumes.
Applications in the UHPLC to HPLC region Area value 1,500,000
require a diverse range of analytical 1,000,000
column sizes. Using a Nexera X2 series 500,000
autosampler allows you to choose the
0
optimal injection volume for the given 0.0 10.0 20.0 30.0 40.0 50.0 60.0
column size, without any compromise in Injection volume (µL)
analytical reliability.
Injection Volume (µL) Repeatability (n = 6) Injection Volume (µL) Repeatability (n = 6)
0.1 0.67% 2 0.09%
0.2 0.32% 5 0.05%
0.5 0.26% 10 0.05%
0.7 0.14% 20 0.04%
1 0.11% 50 0.03%
Injection Repeatability (Actual Values)

Accurate Analysis Even at Volumes Below 1 µL
Nexera X2 series autosamplers enable highly accurate mAU
analysis even at injection volumes below 1 µL. For UHPLC
9 %RSD of Area (n=6)
analysis using small columns, the optimal injection volumes Caffeine:0.35
are correspondingly small as well. 8
Especially when using sample solvents with a high elution 7
strength, it is difficult to increase the injection volume.
6
Normally, that means samples must be diluted to reduce the
5
effects of the sample solvents.
Nexera X2 series autosamplers are capable of highly accurate 4
micro volume injection, allowing even samples with high
3
organic solvent concentrations after pretreatment to be
2
injected directly without dilution.
1
0
0.0 0.5 1.0 1.5 2.0 2.5 3.0 min
Reproducibility Test for 0.1 µL Injection Volume

Easily Accommodates a Large Number of Samples
If a large number of samples needs to be placed at the same
time and their temperature must be kept constant, then an
optional Rack Changer II for the SIL-30AC is especially useful.
This rack changer can hold up to 12 microplates (96/384-well
MTPs or DWPs) or 1.5 mL vial racks, which provides ample
capacity for large numbers of samples.

Rack Changer II

9