Page 8 - Shimadzu Nexera X2

High-Performance Autosamplers

Facilitate Ultra High Sensitivity Analysis

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Popular Low-Carryover Performance Now Further Improved
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Nexera X2 series autosamplers are carefully designed to reduce carryover in increasingly y
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high-sensitivity analytical applications, with features such as a multi-rinse mechanism,
included as standard, that allows you to select from multiple types of solvents. They also
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offer high accuracy at the injection volumes optimal for column sizes typically used in
exera
UHPLC applications. Together with the broad injection volume linearity, this makes Nexera
X2 series autosamplers more than capable of handling applications from UHPLC to HPLC.
LC.
Multi-Rinse Mechanism Eliminates Carryover
When analyzing multiple components simultaneously, they often have significantly different polarities. In such cases, a single rinse
solution is insufficient for adequate rinsing. To solve this problem, Nexera X2 series SIL-30AC and SIL-30ACMP autosamplers feature a
hardware structure that resists adsorption as well as a rinse mechanism that minimizes carryover.
Multi-rinse mechanism supports up to 4 solutions
The needle surface can be rinsed by a maximum of two rinse solutions Sample loop
and the needle inner surface can be rinsed by a maximum of three Needle
rinse solutions. Of course, rinsing with just one solution in the usual
way is also possible. The rinsing order can be set as desired. Rinse ports

Contact surfaces of needle and needle port
where carryover is likely to occur Drain
R0 R1 R2 R3
For rinsing needle surface and For rinsing needle
inner surface (3 types) surface (1 type)
Needle port
(schematic diagram) Multi-rinse mechanism can
accommodate up to 4 solutions
Port section is rinsed by any of the rinse solutions Needle port rinsing mechanism
(1 to 3 solutions)
The needle port is one of the places on an autosampler
where carryover is likely to occur. On the SIL-30ACMP, the
Discharge to drain port needle port is rinsed automatically.

HPLC detectors, such as mass spectrometers, are becoming increasingly sensitive each year. Consequently, the carryover levels required
for HPLC systems are becoming extremely strict. Nexera X2 series SIL-30AC and SIL-30ACMP autosamplers offer the ultimate in
low-carryover performance, due to the thorough carryover-reducing mechanism.

Ultralow carryover achieved without rinsing Ultralow carryover even on a high-sensitivity LC/MS/MS
When rinsing is performed to keep carryover low, the total analysis time Ultralow carryover performance is required with LC/MS systems.
sometimes increases as the number of analyses increases. Nexera The SIL-30ACMP demonstrates exceptional carryover performance even on
autosamplers excel in suppressing carryover even without rinsing. compounds such as chlorhexidine that are very prone to adsorption.
Moreover, the SIL-30ACMP features an improved rinsing mechanism to
achieve even lower carryover.
mAU
5.0 (x10,000)
4.0 2.0
0.0004 % without rinsing Chlorhexidine 500 ng/µL
3.0 1.5 0.0001 %
2.0
1.0
1.0
Caffeine 4 g/L 0.5
0.0
Blank
0.0 Blank
0 1.0 2.0 3.0 4.0 5.0 min 0.00 0.25 0.50 0.75 1.00 min

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