Page 14 - Shimadzu Nexera X2

Improved Efficiency of Non-Analytical Tasks

Autosampler Pretreatment Function Automates Sample Preparation

Creating calibration curves is essential for quantitative analysis, and is a critical process that must not be compromised if concentrations are
to be measured correctly. By using the Nexera X2 SIL-30AC autosampler and Shimadzu workstation, simply place standard stock solution
samples and unknown samples in position, and the system automatically does the rest, from the time-consuming process of preparing
calibration curves to calculating quantitative results. (The SIL-30AC autosampler offers automatic pretreatment capability.)

Prepare sample stock solution Sample stock solution
Prepare standard stock solution 500 Standard Standard Sample
stock solution
stock solution stock solution
Place samples in autosampler
Dilution process
Create calibration curves is automated. 500
Simply place stock solutions
Analyze samples
250 100 50 25 10 5 in the autosampler.
Quantitative calculations Standard solution Sample solution
Dilution process is time-consuming.
Automatically performs everything from creating
calibration curves to calculating quantitative results.
µV
1400000
175000 Caffeine 5 mg/L Peak R = 0.99998
2
Caffeine 10 mg/L 1.Caffeine 1200000
150000 Caffeine 25 mg/L
Caffeine 50 mg/L
125000
Caffeine 100 mg/L
800000
100000 Caffeine 250 mg/L Peak Area 1000000
75000 600000
50000 400000
25000 200000
0 0
0 50 100 150 200 250 300
0.0 1.0 2.0 3.0 4.0 min
Concentration (mg/L)
Chromatograms are obtained from automatically A calibration curve is also created automatically from
diluted standard stock solutions. the resulting chromatograms for standard stock solutions.
Automated Pre-Column Derivatization Reaction
The Nexera X2 SIL-30AC autosampler is also capable of automat- mV
ing pre-column derivatization reactions. Because reaction times 200 22
and reagent amounts added can be kept constant, it enables
150
highly reproducible derivatization reactions. In addition,
automating the process eliminates the time and trouble of 100
12 13 15
derivatization. The analytical example below shows how 22 1 10 11 18 20
4 7 9 17 19
amino acid components were analyzed with excellent sensitivity, 50 2 3 5 8
6 14 16 21
by using two types of reagents to automatically derivatize amino
0
acids and using the automatic wavelength switching function of 0.0 2.5 5.0 7.5 min
the RF-20Axs fluorescence detector. Peaks
1. Asparti Acid 2. Glutamic Acid 3. Asparagine 4. Serine 5. Glutamine 6. Histidine
7. Glycine 8. Threonine 9. Citrulline 10. Arginine 11. Alanine 12. GABA 13. Tyrosine
Analytical Conditions
14. Cys-Cys 15. Valine 16. Methionine 17. Tryptophan 18. Phenylalanine19. Isoleucine
20. Leucine 21. Lysine 22. Proline
Column : YMC-Triart C18 1.9 µm
(75 mmL. × 3.0 mmI.D., 1.9 µm, YMC Co., Ltd.) Repeatability
Mobile Phase : A : 20 mmol/L Phosphate Potassium Buffer (pH 6.9)
B : 45/40/15 Acetonitrile/Methanol/Water Area%RSD Area%RSD
Time Program : B Conc.11 % 13 % (0.00−3.00 min) 31 % (5.00 min) Asp 0.50 GABA 0.41
37 % (7.5 min) 70 % (10.00 min) Glu 0.48 Tyr 0.55
100 % (10.50-13.50 min) 11 % (14.00 min) Asn 0.51 Cys-Cys 0.46
Flow Rate : 0.8 mL/min Ser 0.41 Val 0.71
Column Temp. : 35°C Gln 0.56 Met 0.71
Injection Volume : 1 µL
Detection : RF-20AXS Ex. at 350 nm, Em. at 450 nm His 0.57 Trp 0.70
Ex. at 266 nm, Em. at 305 nm (9.0 min) Gly 0.29 Phe 0.73
Cell Temp. : 20°C Thr 0.55 Ile 0.63
Flow Cell :Conventional Cell Citrulline 0.46 Leu 0.55
Arg 0.45 Lys 0.56
Ala 0.46 Pro 2.35
Note: For more details, refer to Application News (L432).
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