Page 18 - Shimadzu Nexera X2

Revolutionary Spectral Analysis Processing

Complete Separation of Co-eluted Peaks by i-PDeA (intelligent Peak Deconvolution Analysis, patent pending)
i enables the extraction of a single peak from co-eluted peaks by utilizing differences in spectra. This new separation method
-PDeA
i
-PDeA
removes discussion of integration methods for co-eluted peaks. The also helps detect impurity peaks in a target peak.

Step 2 Step 3
Step 1
Extraction of single peak signal using Display of single component
Analysis
the difference between each spectrum representative peak
AU : 1
: 2
Component B Component A Component B
Component A

nm
1 2
1 shows extraction of component B.
shows extraction of component A.
2
Baseline separation Vertical separation
Removes peak integration ambiguities

Example 1: Remove tailing peak
Tailing peak 0.5 mg/L spiked
0.488 mg/L measured
(98% accuracy)
i
Target peak -PDeA

0.00 0.25 0.50 0.75 1.00 1.25 min 0.00 0.25 0.50 0.75 1.00 1.25 min
The target peak is on the tailing peak Remove the tailing peak!!

Example 2: Impurity peak extracted from co-eluted peak

Peak purity curve

i -PDeA

0.22 0.24 0.26 0.28 min 0.22 0.24 0.26 0.28 min
This peak looks a single peak. By removing the main peak, the impurity peak appears on the chromatogram!!

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