Page 17 - Shimadzu Nexera Prep

Shim-pack Scepter Columns

Excellent Stability & Performance using a Wide Range of LC Conditions

Shim-pack Scepter LC columns, which are the next generation of organic silica hybrid-based columns, are designed for stability
and performance in a wide range of mobile phase conditions. With different chemistry characteristics, Shim-pack Scepter
columns are effective for method development/scouting under conditions that may compromise traditional silica-based columns.

With different particle sizes (1.9 µm, 3 µm, 5 µm) and different column dimensions, Shim-pack Scepter LC columns are fully
scalable between UHPLC, HPLC and preparative LC, making method transfer seamless between different laboratory
instrumentation.

Reversed Phase
C18 HD-C18 C8 Phenyl PFPP
Trifunctional C18 Trifunctional C18 Trifunctional Trifunctional
Functional Group Trifunctional C8
Generic Purpose Type High Density Type Phenylbutyl Pentafluorophenylpropyl
Particle Organic Silica Hybrid
Particle Size 1.9 µm, 3 µm, 5 µm
Pore Size 12 nm 8 nm 12 nm
End Capping Proprietary None
pH Range 1 – 12 1 – 10 1 – 8
100% Aqueous Condition Yes No No Yes Yes
USP Classification L1 L1 L7 L11 L43

Example of Scaling Up from Analytical to Semi-Preparative Work

This is an example of scaling up in which seven types of
parabens are targeted using a 150 mm long column with a 1 Analytical Scale 1 mL/min
particle size of 5 µm. 2 3 4
5
6 7
The gradient elution conditions investigated at the analytical
scale are transitioned to the semi-preparative scale. A
comparable chromatogram is obtained at both scales.
Preparative Scale 19 mL/min
Column: Shim-pack Scepter C18-120 (4.6 mm × 150 mm, 5 µm)
Column: Shim-pack Scepter C18-120 (20 mm × 150 mm, 5 µm)

0.0 5.0 10.0 15.0 min
1: Metylparabene 5: Pentylparabene
2: Ethylparabene 6: Heptylparabene
3: Propylparabene 7: Nonylparabene
4: Butylparabene
Example of Scaling Up for Parabens

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