Page 11 - Shimadzu Nexera Prep

Flow Splitter for Fractionation Triggered by LC/MS Signals

For fractionation triggered by LC/MS signals, a portion of the sample must be introduced into the LC-MS.
The dedicated flow splitter splits a small portion of the flow from the preparative stream to the MS stream. This enables the use
of LC-MS detection signals for triggering fractionation while maintaining a high recovery rate.

MS stream
Preparative stream

Make up pump
Drain Mass
spectrometer

Flow Splitter
Column

Preparative pump
UV detector
Liquid handler

By splitting the stream into two after separation by the column, a large
portion is introduced to the fraction collector side and a small portion
to the MS side.

Nexera Prep LC/MS Preparative System

Customized Detection Methods

Signals from various detectors can be used to trigger the fractionation. The optimal system configuration can be obtained for
different samples and conditions.

UV-VIS Detector SPD-40 Refractive Index Detector Evaporative Light Scattering Detector Mass Spectrometer
PDA Detector SPD-M40 RID-20A ELSD-LT III LCMS-2050

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