Page 10 - Shimadzu Nexera Prep

Preparative Work for

Non-UV Absorptive Components

Capable of High Purity Preparation Triggered by Up to Four Detector Channel Signals
Nexera Prep LC/MS Preparative System

Using MS Signal Triggers Enables Recovery with No Target Fraction Omissions

It can be difficult to prepare low UV absorptive components using just a UV signal as the trigger, so there is a risk that the fraction
will be missed. By using the MS signal as the trigger, the preparative work can be performed simply, with nothing missed. By specify-
ing the m/z of the target component, fractions can be collected with confidence. The LCMS-2050 mass spectrometer enables
high-sensitivity and high-resolution detection for preparative work with no target components omitted.

UV Detection Chromatogram

0.0 10.0 20.0 30.0 min
Reliable Preparation of Low UV
MS Detection Chromatogram Absorptive Components

m/z 255 (+)
m/z 247 (+)
m/z 441 (+)
m/z 233 (+)
m/z 487 (+)
m/z 272 (+)
0.0 10.0 20.0 30.0 min

Purification of Un-separated Target Components

Two specific m/z values (from the target component and its impurity) were simultaneously used to obtain a “high-purity” fraction,
even in the case of incomplete chromatographic separation.

Target Component A

Impurity B

Ch: 1
Ch: 2

High Purity Recovery of Target Component A
(Using two MS signals as triggers)
The MS signal that detects the target component A is used to trigger the start of the fractionation.
The MS signal that detects impurity B is used to stop the fractionation.

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