Page 8 - Shimadzu Nexera Prep

Preparative Work for Target Components at

High Purity Levels and High Concentrations

Automatic Removal of Non-volatile Salts

In the conventional preparative LC, salts derived from the mobile phase are included in the recovered product. With Nexera UFPLC,
salts derived from the mobile phase can be removed on the trap column.
In the picture below, Ibuprofen was prepared using a solvent containing ammonium chloroacetate, a non-volatile salt. With
conventional preparative LC, ammonium chloroacetate precipitated at the same time during evaporation. However, ibuprofen was
recovered as a single component with Nexera UFPLC due to the use of a trap column.

mAu
400 Prep LC
Trapping column 1
Trapping column 2 Valerophenone
300
Trapping column 3
200 Ammonium Chloroacetate Salts

Ibuprofen Nexera UFPLC
100

0
0.0 2.5 5.0 7.5 min
Preparative Chromatogram for Ibuprofen and Ibuprofen
Analogous Substances (Nexera UFPLC)

Concentration and Powdering of Target Ingredients in a Short Period of Time

Using Nexera UFPLC, samples are repeatedly injected and the target components are introduced into the same trap column,
allowing concentration on the trap column (up to 100 mg capacity). After concentration, the target component is eluted with an
organic solvent, allowing recovery of the target component at a high concentration and shortening the time for evaporation.
The volume of recovered liquid and the time required for evaporation were measured when 100 mg of the target component,
ibuprofen, was puri ed by trap puri cation. Compared to the conventional preparative LC process, the overall time was reduced
by 50%.
Comparison of Preparative LC and UFPLC Fractionation
Fraction vol. Fraction conc. Drying time * 1
System
(mL) (mg/mL) (min)
Typical Preparative LC 93.0 1.1 355 * 2
Nexera UFPLC 9.1 11.0 130
*1 Comparison of drying times when a centrifugation enrichment dryer is used
*2 Time for drying the solution (20 mg) collected in one cycle

15 min × 5 cycles 355 min
Typical
Preparative Preparative Work Powderization Total:
LC 430 min
15 min × 5 cycles 15 min 130 min
Nexera Preparative Work Powderization Total: 220 min
UFPLC
Enrichment Purification
Comparison of Procedural Times for Typical Preparative LC and UFPLC

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