Page 99 - Shimadu Consumables Catalog

Analysis of Amino Acids

Shim-pack Amino Series
Examples of Cation Analysis

Shim-pack Amino series uses polystyrene gel as solid support, making it possible to utilize both electrostatic reaction and hydrophobic
reaction. It is ideal for the analysis of amino acids.

Analysis of Amino Acid Standard Analysis of Vinegar
Conditions Peaks
Column : Shim-pack Amino-Na (100 mmL × 6.0 mmI.D., 5 μm) 1. ASP 6. GLY 11. ILE 16. HIS
(P/N: 228-18837-91) 2. THR 7. ALA 12. LEU 17. LYS
Mobile Phase : Mobile phase kit for amino acid analysis (Na Type) 3. SER 8. CYSTINE 13. TYR 18. ARG
9. VAL
14. PHE
4. GLU
(P/N: 228-21195-94) 5. PRO 10. MET 15. GABA
Flow Rate : 0.4 mL/min
Col. Temp. : 60 °C
Detection : RF (Post-column derivation)

Analysis of Soy Sauce

Simultaneous Analysis of 38 Amino Acids
Conditions
Column : Shim-pack Amino-Li (100 mmL × 6.0 mmI.D., 5 μm)
(P/N: 228-18837-92)
Mobile Phase : Mobile phase kit for amino acid analysis (Li Type)
(P/N: 228-21195-95)
Flow Rate : 0.6 mL/min
Col. Temp. : 39 °C
Detection : RF-10AXL Ex. 350 nm, Em. 450 nm

Product Information

Particle Size Dimensions
Column Stationary Phase P/N Guard Column
(μm) (Length × I.D., mm)
Shim-pack AMINO-NA Na type sulfone group 5 100 × 6.0 228-18837-91 228-18837-93 *
Shim-pack AMINO-LI Li type sulfone group 5 100 × 6.0 228-18837-92 -
* Dedicated for the analysis of cyanide. Please do not use it in the analysis of amino acids.
In the analysis of amino acids, the following trap columns are required.
Dimensions
Description P/N
(Length × I.D., mm)
ISC-30/S 0504 NA (For trapping Na type ammonia) 50 × 4.0 228-14206-91
ISC-30/S 0504 LI (For trapping Li type ammonia) 50 × 4.0 228-00821-91

Pg A-95