Page 97 - Shimadu Consumables Catalog

Analysis Examples
Tocopherol Isomers Phospholipid Classes

Isomers and structurally similar compounds that are difficult to
separate can be analyzed by Nexera UC and Shim-pack UC series
at a high speed. In the example below, four kinds of tocopherol
isomers were analyzed by Nexera UC and Shim-pack
UC Sil. As shown below, analysis time was reduced to
one-third the time analysis takes with a conventional HPLC
method while resolution was improved.

The example above shows the result of the analysis of
phospholipid classes using Shim-pack UC Diol with Nexera UC.
The ODS-based Shim-pack UC GIS II can also be used to analyze
molecular species of phospholipids with the same modifiers.
Nexera UC and Shim-pack UC series are ideal for analyzing
complex samples.

Comparison of retention and resolution of tocopherol isomers
between LC conditions and SFC ones

Preparative Columns

Particle Size I.D. (mm)
Column 10 20 28
(μm) Length (mm)
Shim-pack UC-HyP 5 250 227-32600-03 227-32600-04 227-32600-05
Guard Column 5 20 227-32600-06 - -
Shim-pack UC-Py 5 250 227-32601-03 227-32601-04 227-32601-05
Guard Column 5 20 227-32601-06 - -
Shim-pack UC-PBr 5 250 227-32602-03 227-32602-04 227-32602-05
Guard Column 5 20 227-32602-06 - -
Shim-pack UC-Choles 5 250 227-32603-03 227-32603-04 227-32603-05
Guard Column 5 20 227-32603-06 - -
Shim-pack UC-PyE 5 250 227-32604-03 227-32604-04 227-32604-05
Guard Column 5 20 227-32604-06 - -
Shim-pack UC-Triazole 5 250 227-32605-03 227-32605-04 227-32605-05
Guard Column 5 20 227-32605-06 - -

Shim-pack UC-Sil II 5 250 227-32607-03 227-32607-04 227-32607-05
Guard Column 5 20 227-32607-06 - -
Shim-pack UC-Diol II 5 250 227-32606-03 227-32606-04 227-32606-05

Pg A-93