Page 102 - Shimadu Consumables Catalog

Dedicated Columns

Shim-pack Fast-OA

High-speed analysis of organic acids
Shim-pack Fast-OA columns are packed with an ion-exclusion polymer, and utilizing an acidic mobile phase, can separate mixture
solutions based on the pKa value of each sample component. The Shim-pack Fast-OA is a column for the high speed analysis of
organic acids. It can separate multiple organic acids in a short time and supports real-time monitoring of their concentration levels.
Analysis Examples
Analysis of Short-Chain Fatty Acids Improved Separation: Columns in Series
Peaks Peaks
1. Pyruvic acid 1. Lactic acid
2. Succinic acid 2. Formic acid
3. Formic acid
4. Acetic acid
5. Propionic acid
6. Butyric acid
7. Valeric acid

Because the column design is optimized for the high-speed analysis of organic acids, even short-chain fatty acids such as valeric acid
are eluted within 10 minutes. If separation with one column is not enough, up to three Shim-pack Fast-OA columns can be connected
in series to improve the separation

Conditions
Column : Shim-pack Fast-OA (P/N: 228-59942-41) + Shim-pack Fast-OA (G) (P/N: 228-59942-42)
Mobile Phase : 5 mmol/L p-Toluenesulfonic acid
pH buffering solution : 5 mmol/L p-Toluenesulfonic acid, 20 mmol/L Bis- Tris, 0.1 mmol/L EDTA
Flow Rate : 0.8 mL/min
Detection : Conductivity Detector (CDD-10AVP)

Product Information

Product Name Length x I.D. Part Number
Shim-pack Fast OA 100 mm x 7.8 mm 228-59942-41
Guard column + Holder 10 mm x 4.0 mm 228-59942-42
Guard column (4 pcs) 10 mm x 4.0 mm 228-59942-43

Pg A-98