Page 100 - Shimadu Consumables Catalog

Dedicated Columns

Analysis of Sugar and Organic Acid

Shim-pack SCR Series
Shim-pack SCR-101N/C/P are suitable for the analysis of monosaccharides. Since the samples are separated under a mixed mode of gel
filtration and ligand exchange, the selectivity differs depending on the type of cation.
Shim-pack SCR-101H and SCR-102H are ion exclusion chromatography columns, using H type sulfonated styrene polymer as stationary
phase. They are ideal for analysis of organic acids using an acid aqueous solution (e.g. aqueous solution of perchloric acid) as mobile
phase.

Peaks
1. PEG 4000
2. Maltose
3. Glucose
4. Fructose
5. Glycerol
6. Ethanol
7. Sorbitol
Conditions
Column : Shim-pack SCR-101C (300 mmL × 7.9 mmI.D., 10 μm) (P/N: 228-17889-91)
Mobile Phase : Water
Flow Rate : 1.0 mL/min
Col. Temp. : 80 °C
Detection : RID
Analysis of Saccharide Standard

Peaks
1. Phosphoric acid 7. Lactic acid
2. α-Ketoglutaric acid 8. Formic acid
3. Citric acid 9. Fumaric acid
4. Pyruvic acid 10. Acetic acid
5. Malic acid 11. Levulinic acid
6. Succinio acid 12. Pyroglutamic acid
Conditions
Column : Shim-pack SCR-102H (2 columns in series) (P/N: 228-17893-91)
Mobile Phase : 5 mM p-Toluene sulfonic acids aqueous solution
Flow Rate : 0.8 mL/min
Col. Temp. : 40 °C
Detection : CDD (pH buffer organic acids analysis system)
Analysis of Organic Acids
Product Information

Particle Size Dimensions
Column Stationary Phase P/N Guard Column
(μm) (Length × I.D., mm)
Shim-pack SCR-101N Na type sulfone group 10 300 × 7.9 228-07730-92 228-09619-92
Shim-pack SCR-101C Ca type sulfone group 10 300 × 7.9 228-17889-91 228-17891-91
Shim-pack SCR-101P Pb type sulfone group 10 300 × 7.9 228-17890-91 228-17892-91
Shim-pack SCR-101H H type sulfone group 10 300 × 7.9 228-07730-93 228-09619-93
Shim-pack SCR-102H H type sulfone group 7 300 × 8.0 228-17893-91 228-17924-91

Pg A-96