Page 103 - Shimadu Consumables Catalog

Analysis of Nucleotides, Oligonucleotides and Protein

Shim-pack WAX/WCX Series
Shim-pack WAX/WCX series columns are chemically-bonded hydrophilic silica gel based ion exchange columns. Shim-pack WAX-1 is
ideal for analysis of nucleotides and oligonucleotides while Shim-pack WAX-2 and WCX-1 is ideal for analysis of proteins.

Peaks
1. UMP 7. ADP
2. CMP 8. UTP
3. AMP 9. GDP
4. GMP 10. CTP
5. UDP 11. ATP
6. CDP 12. GTP
Conditions
Column : Shim-pack WAX-1 (50 mmL × 4.0 mmI.D., 3 μm) (P/N: 228-16225-91)
Mobile Phase : A) 20mM phosphate buffer solution (pH 7)
B) 480mM phosphoric acid buffer solution (pH 6.85)
gradient elution
Flow Rate : 1.0 mL/min
Col. Temp. : 45 °C
Detection : UV 260 nm
Analysis of Mononucleotides
Peaks
1. Ovalbumin
2. Myoglobin
3. α-Chymotrypsinogen A
4. Ribonuclease A
5. Lysozyme
Conditions
Column : Shim-pack WCX-1 (50 mmL × 4.0 mmI.D., 5 μm) (P/N: 228-16366-91)
Mobile Phase : A) 20mM phosphate buffer solution (pH 6.0)
B) Sodium sulfate
gradient elution
Flow Rate : 1.0 mL/min
Col. Temp. : Ambient
Detection : UV 415 nm
Analysis of Protein Standard

Product Information

Particle Size Dimensions
Column Stationary Phase P/N
(μm) (Length × I.D., mm)
Shim-pack WAX-1 Tertiary amino group 3 50 × 4.0 228-16225-91
Shim-pack WAX-1 Tertiary amino group 3 150 × 4.6 228-16225-92
Shim-pack WAX-1T Tertiary amino group 3 50 × 4.6 228-18257-91
Shim-pack WAX-2 Tertiary amino group 5 50 × 4.0 228-16365-91
Shim-pack WCX-1 Carboxyl group 5 50 × 4.0 228-16366-91
Pre-column Diol * Diol group 10 50 × 4.0 228-16367-91
* Installed between the liquid pump and the sample injector to protect the column.

Pg A-99