Page 93 - Shimadu Consumables Catalog

Mastro SP
TM
Ion-paring reagents are required to analyze the highly-polar compound such as phosphate groups. However, ion-paring reagents are
not ideal for LCMS applications as they as difficult to remove from the flow lines. Mastro TM SP columns are ideal for the analysis of
such compounds without the use of ion-paring reagents.

Analytical condition
Instruments : Shimadzu Nexera/LCMS-8050
Column : Mastro™ SP (2.1 mmI.D × 100 mmL., 5 μm)
Mobile phase : A) 10 mM Ammonium acetate/ACN=90/10
Mobile phase : B) 50 mM Ammonium acetate/ACN=80/20
0% B (0-4.0 min) 60% B (9.0 min)
00% B (11.0-20.0 min)
0% B (20.1-25.0 min)
T. Flow : 0.5 mL/min
Nebulizing gas : 3.0 L/min
Drying gas : 12 L/min
DL Temp. : 250 ºC
BH Temp. : 400 ºC
Injection Vol. : 3 μL

Particle Size I.D (mm) Length
(μm) 100 mm
5.0 2.1 SSOT-G10005

Mastro2™ PFP

Mastro2 PFP is the column packed with PFP (Penta Fluoro Phenyl) that has high retention capability. Generally, PFP columns have
efficiency for retaining and separating regioisomers and halogen compounds that are difficult to be analysed with C18 column.

Separation of cresol
Analytical conditions
Mastro2™ PFP Typical PFP
Instrument : NexeraX2_SPD20A(Semi-micro cell) α2,3 1.06 1.10
Column : Mastro2™ PFP (150 mmL. × 2.0 mm I.D. 3 μm)
Typical PFP* (150 mmL. × 2.1 mm I.D. 3 μm) α3,4 1.10 1.82
1
Mobile phase : H2O / CH 3OH = 60 / 40 Rs1 1.50 0.59
Flow rate : 0.2 mL/min Rs2 2.50 0.92
Detection : UV 254 nm
Column oven : 40 ºC
Sample : 1. Uracil, 2. p-Cresol, 3. m-Cresol, 4. o-Cresol
* Metal free column
1

Mycotoxin components (3-ADON, 15-ADON) analysis

Analytical conditions

Instrument : NexeraX2_SPD20A (Semi-micro cell)
Column : Mastro2™ PFP (150 mm L, × 2.0 mm I.D. 3 μm)
Mobile phase : A) 10 mM CH 3COONH4 in H2O
B) 2% CH3COOH in CH 3OH
Gradient condition
Flow rate : 0.4 mL/min
Detection : UV 254 nm
Column oven : 40 ºC
Sample : 1. 15-ADON, 2. 3-ADON

Particle Size I.D (mm) Length
(μm) 100 mm 150 mm
3.0 2.0 370-01015-64 370-01015-84

Pg A-89