Page 89 - Shimadu Consumables Catalog

Shim-pack Diol Series

Shim-pack Diol series is a kind of gel filtration chromatography (GFC) column. GFC is used to separate water-soluble high polymers
such as polysaccharides, proteins, and nucleic acids according to their molecular sizes by using hydrophilic packing materials and
aqueous mobile phase.
Shim-pack Diol series is packed with porous spherical silica gel chemically bonded with a hydroxyl group. Due to the hydrophilic
hydroxyl group, Shim-pack Diol series can be used in high-speed GFC and provide sharp peaks during the analysis of protein and
biochemicals (such as enzymes).

Product Information

Particle Size Dimensions
Column Stationary Phase P/N
(μm) (Length × I.D., mm)
250 × 7.9 228-14775-91
Shim-pack Diol-150 Diol group 5
500 × 7.9 228-14775-92
250 × 7.9 228-14776-91
Shim-pack Diol-300 Diol group 5
500 × 7.9 228-14776-92
Pre-column Diol * Diol group 10 50 × 4.0 228-16367-91
* Installed between the liquid pump and the sample injector to protect the Shim-pack Diol column.

Analysis Examples

Calibration Curves

Peaks
1. Blue dextran 2000
2. Thyroglobulin
3. y-Globulin
4. Transferrin
5. Human serum albumin
6. Ovalbumin
7. β-Lactoglobulin
8. Chymotrypsin
9. Myoglobin
10. Lysozyme
11. Ribonuclease A
12. Cytochrome C
13. Gly-Tyr
Conditions
Column : Shim-pack Diol Series (500 mmL × 7.9 mmI.D., 5 μm)
Mobile Phase : A) 10mM phosphate buffer solution (pH 7)
B) 0.2M sodium sulfate
Flow Rate : 1.0 mL/min
Col. Temp. : Ambient
Detection : UV 280 nm

Analysis of Protein Standard

Peaks Conditions
1. Glutamate dehydrogenase Column : Shim-pack Diol-300 (500 mmL × 7.9 mmI.D., 5 μm)
2. Lactate dehydrogenase (P/N: 228-14776-92)
3. Enolase Mobile Phase : A) 10mM phosphate buffer solution (pH 7)
4. Adenylate kinase B) 0.1M sodium chloride
5. Cytochrome C
Flow Rate : 1.0 mL/min
Col. Temp. : Ambient
Detection : UV 280 nm

Pg A-85