Page 85 - Shimadu Consumables Catalog

TM
Various molecules give an increase in the peak intensity on Nexera Mikros LC-MS/MS platform with an MC PLONAS column.

Shim-pack MC PLONAS C18

3000 6.0e2

2500 5.0e2
Micro
Semi-micro
2000 4.0e2

1500 3.0e2
Aldosterone 1 pg/mL
1000
2.0e2
500
1.0e2

0
0.0e0
1.02 .0 3.04 .0 5.0 12 34 5( min)
Column : Shim-pack MC PLONAS C18 (0.2 mm I.D. x 100 mm L., 2.7 μm)
*Semi-micro data acquired with the 2.1 mm I.D. column which has the same chemical profile as an MC PLONAS column.
Mobile phase : A: Water, B: Methanol, gradient elution
Flow rate: : 4 μm/min
Temperature : 50 °C
Injection volume : 1 μL
MS detection : LCMS-8060, with Micro-ESI source, ESI (-)

Shim-pack MC PLONAS C8

1. All-trans Retinol (A) 6
2. Epoxy-Menaquinone-4 (epoxy-MK-4) 5
3. Menaquinone-4 (MK-4) 1
4. -Tocopherol (E) 4
5. Epoxy-Phylloquinone (epoxy-K1) 7
6. Phylloquinone (K1)
7. Menaquinone-7 (MK-7)
3
2

Column : Shim-pack MC PLONAS C8 (0.2 mm I.D. x 100 mm L., 2.7 μm)
Mobile phase : A: Ammonium acetate aq.
B: Ammonium acetate in methanol, gradient elution
Flow rate : 4 μL/min
Temperature : 50 °C
Injection volume : 1 μL
0.0 1.02 .0 3.04 .0 5.06 .0 7.08 .0 9.0 10.0 11.0 12.0 13.0m in MS detection : LCMS-8060, with Micro-ESI source, ESI (-)
20 pg on column
* each peak is in full scale intensity

Pg A-81