Page 85 - Shimadu Consumables Catalog
P. 85

TM
           Various molecules give an increase in the peak intensity on Nexera Mikros  LC-MS/MS platform with an MC PLONAS column.

              Shim-pack MC PLONAS C18

           3000                                               6.0e2


           2500                                               5.0e2
                                         Micro
                                         Semi-micro
           2000                                               4.0e2

           1500                                               3.0e2
                                                                                 Aldosterone 1 pg/mL
           1000
                                                              2.0e2
           500
                                                              1.0e2

            0
                                                              0.0e0
                     1.02    .0     3.04   .0     5.0                    12            34             5(   min)
           Column        : Shim-pack MC PLONAS C18 (0.2 mm I.D.   x 100 mm L., 2.7 μm)
                           *Semi-micro data acquired with the 2.1 mm I.D. column which has the same chemical profile as an MC PLONAS column.
           Mobile phase   : A: Water, B: Methanol, gradient elution
           Flow rate:     : 4 μm/min
           Temperature   : 50 °C
           Injection volume   : 1 μL
           MS detection   : LCMS-8060, with Micro-ESI source, ESI (-)

              Shim-pack MC PLONAS C8

            1. All-trans  Retinol (A)            6
            2. Epoxy-Menaquinone-4 (epoxy-MK-4)  5
            3. Menaquinone-4 (MK-4)  1
            4.  -Tocopherol (E)                4
            5. Epoxy-Phylloquinone (epoxy-K1)          7
            6. Phylloquinone (K1)
            7. Menaquinone-7 (MK-7)
                                              3
                                             2




                                                              Column          : Shim-pack MC PLONAS C8 (0.2 mm I.D.   x 100 mm L., 2.7 μm)
                                                              Mobile phase     : A: Ammonium acetate aq.
                                                                              B: Ammonium acetate in methanol, gradient elution
                                                              Flow rate          : 4 μL/min
                                                              Temperature       : 50 °C
                                                              Injection volume : 1 μL
           0.0  1.02  .0  3.04  .0  5.06  .0  7.08  .0  9.0  10.0 11.0 12.0 13.0m in  MS detection      : LCMS-8060, with Micro-ESI source, ESI (-)
            20 pg on column
            * each peak is in full scale intensity


















                                                                                                         Pg A-81
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