Page 80 - Shimadu Consumables Catalog

Bio LC Columns

Shim-pack Bio LC Columns

Solution for Analysis of Peptides, Oligonucleotides and Biopharmaceuticals

The accurate analysis of biopharmaceuticals compounds is required for developing higher quality biopharmaceuticals. Shim-pack Bio
Diol and IEX columns will improve the accuracy of the characterization of peptides, oligonucleotide and biopharmaceuticals.

Shim-pack Bio Diol : Size Exclusion Chromatography Columns

With different pore sizes, Shim-pack Bio Diol LC columns are effective for analysis of aggregates and fragments of mAb,
oligonucleotides and carbohydrates.

Rapid mAb Aggregate Analysis using 2μm Shim-pack Diol-300 column
Shim-pack Diol-60 Diol-120 Diol-200 Diol-300
Bio Diol
Particle Silica
Ligand Dihydroxypropyl(Diol)
Particle Size 3 μm, 5 μm 2 μm, 3 μm, 5 μm
Pore Size 6 nm 12 nm 20 nm 30 nm
pH Range 5.0 - 7.5
below 1,000 - 5,000 - 20,000 -
Molecular
10,000 100,000 300,000 1,000,000
By reducing the particle size from 5 μm to 2 μm, the resolution between
aggregates and monomers was greatly improved. Furthermore, by
reducing the column length from 300 mm to 150 mm using a 2 μm
particle, 50% less run time was achieved, while maintaining resolution
as compared to the original method with a 5 μm, 4.6×300 mm column.
Conditions
Column : Shim-pack Bio Diol-300
Eluent : 0.1 M KH2PO4-K2HPO4 (pH 7.0) with 0.2 M NaCl
Flow rate : 0.2 mL/min
Column Temp. : Ambient
Detection : UV 280 nm
Sample : Humanized monoclonal IgG1

Shim-pack Bio IEX : Ion Exchange Chromatography Columns
Shim-pack Bio IEX Columns are available in Q (quaternary ammonium) and SP (sulfopropyl) chemistries and are based on porous
(Q and SP columns) and non-porous (Q-NP and SP-NP columns) hydrophilic polymers with low nonspecific adsorption. The porous
particles offer excellent binding capacity with exceptionally high efficiency and the non-porous particles offer high efficiency and
exceptional resolution.

Rapid mAb Aggregate Analysis using 2μm Shim-pack Diol-300 column Shim-pack
Bio IEX Q-NP SP-NP Q SP
hydrophilic non-porous hydrophilic porous
Particle
polymer polymer
Particle Size 3 μm, 5 μm 5 μm
Ligand - CH N (CH ) - (CH ) SO - - CH N (CH ) - (CH ) SO -
+
+
2 3 3 2 3 3 2 3 3 2 3 3
pH Range 2 - 12
Conditions
Column : Shim-pack Bio IEX Q-NP 5 μm, 4.6×100 mm
(P/N : 227-31003-03)
Mobile Phase A : 10 mM NaOH
Mobile Phase B : 10 mM NaOH with 1.0 M NaClO4
Gradient : 25 55%B (0-15 min), 100%B (15-20 min)
Flow Rate : 1.0 mL/min
Column Temp. : 25 °C
Detection : UV 260 nm
Inj. Volume : 4 μL (5 nmol/mL)

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