Page 92 - Shimadu Consumables Catalog

Metal Free Columns

Mastro™ LC Series

High pressure tolerance metal free column, best solution for chelating compound

Mastro2 C18 Mastro2 PFP Mastro SP
Mode ODS PFP ODS + SAX
Particle Size 2.2 um, 5.0um 3.0 um 5.0 um
Max. Press. 40 MPa (2.2 um), 40 MPa (100 mm), 20 MPa
15 MPa (5.0 um) 50 MPa (150 mm)
Max. Temp. 80 °C ( ) 40 °C
*1
*2
pH range 2 to 7.5 ( ) 2.5 to 7.5
I.D. (mm) 2.0 2.1
Length (mm) 50, 100, 150 100, 150 100
( *1 ) When mixtures of water or acidic aqueous solution (pH 3 or greater) and acetonitrile are used.
( *2 ) Optimum lifetime is obtained at pH 2.5 – pH 7.0 and at 40ºC or less when a buffer is used.
Features

TM
Manufactured with polymer lining and inlet/outlet frits, Mastro range of LC
columns are an excellent choice for compounds which have phosphate group, or
compounds which chelate binding to metal.
Mastro LC columns is controlled activated of stainless by special internal coating.
TM
Highly recommended for LCMS applications in which inertness is critical.

Mastro2 C18
TM
Comparing the SUS body column, the inert body column of other companies, and the Mastro2 ™ column using a phosphate group-
containing compound, not only the area value of Mastro2 ™ increases, but also the variability is extremely small from the obtained
CV value. In addition, since the peak shape is good, the S / N is large and the detection sensitivity is improved.

Analytical condition
Instruments : Nexera Bio
Column : Mastro2 ™ or Typical C18 (150 mmL. X 2.0 mm ID)
Competitor (150 mmL. X 2.1 mm ID)
Mobile phase : 10 mM HCOONH in H O / CH CN = 95/5
3
4
2
Flow rate : 0.2 mL / min
Detection : UV 254 nm
Column Oven : 40ºC
Injection vol. : 1 µL
Unlike other products, Mastro2 ™ has a structure that does not generate metal chelates because all liquid contact parts are PEEK resin.
In addition, a line-up of 2.2 µm microparticles is introduced as the PEEK resin parts are designed to be resistant to deformation even
under high pressure.

Particle Size I.D (mm) Length
(μm) 50 mm 100 mm 150 mm
2.2 370-01003-44 370-01003-64 370-01003-84
2.0
5.0 370-01007-44 370-01007-64 370-01007-84

Pg A-88